Generation of mouse bone marrow-derived macrophages

Manzanero, Silvia (2012). Generation of mouse bone marrow-derived macrophages. In Robert B. Ashman (Ed.), Leucocytes: Methods and protocols (pp. 177-181) New York, NY, U.S.A.: Humana Press. doi:10.1007/978-1-61779-527-5_12


Author Manzanero, Silvia
Title of chapter Generation of mouse bone marrow-derived macrophages
Title of book Leucocytes: Methods and protocols
Place of Publication New York, NY, U.S.A.
Publisher Humana Press
Publication Year 2012
Sub-type Chapter in textbook
DOI 10.1007/978-1-61779-527-5_12
Open Access Status Not Open Access
Series Methods in Molecular Biology
ISBN 9781617795268
1617795267
9781617795275
1617795275
ISSN 1064-3745
1940-6029
Editor Robert B. Ashman
Volume number 844
Chapter number 12
Start page 177
End page 181
Total pages 5
Total chapters 20
Language eng
Formatted Abstract/Summary
Isolation of resident macrophages from mouse tissues involves complex procedures for a small yield. This is inconvenient for many functional macrophage assays, which require large numbers of relatively homogeneous cells. An alternative method is the culture of bone marrow cells in vitro with appropriate growth factors, to allow the differentiation of precursor cells into large numbers of macrophages. This procedure is easy and inexpensive except for the use of M-CSF, the macrophage colony stimulating factor, and it is characterised by high yield and reproducibility. Once obtained, bone marrow-derived macrophages (BMMs) can be used for a considerable number of functional and structural assays and are commonly regarded as a model for the role of resident macrophages in the innate immune system.
Keyword Bone marrow
Macrophage
Mouse
Macrophage colony stimulating factor
CSF-1
Innate
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Book Chapter
Collections: Non HERDC
School of Biomedical Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 20 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 23 May 2012, 19:30:22 EST by Bacsweet Kaur on behalf of School of Biomedical Sciences