Generation of mouse bone marrow-derived macrophages

Manzanero, Silvia (2012). Generation of mouse bone marrow-derived macrophages. In Robert B. Ashman (Ed.), Leucocytes: Methods and protocols (pp. 177-181) New York, NY, U.S.A.: Humana Press. doi:10.1007/978-1-61779-527-5_12

Author Manzanero, Silvia
Title of chapter Generation of mouse bone marrow-derived macrophages
Title of book Leucocytes: Methods and protocols
Place of Publication New York, NY, U.S.A.
Publisher Humana Press
Publication Year 2012
Sub-type Chapter in textbook
DOI 10.1007/978-1-61779-527-5_12
Open Access Status Not Open Access
Series Methods in Molecular Biology
ISBN 9781617795268
ISSN 1064-3745
Editor Robert B. Ashman
Volume number 844
Chapter number 12
Start page 177
End page 181
Total pages 5
Total chapters 20
Language eng
Formatted Abstract/Summary
Isolation of resident macrophages from mouse tissues involves complex procedures for a small yield. This is inconvenient for many functional macrophage assays, which require large numbers of relatively homogeneous cells. An alternative method is the culture of bone marrow cells in vitro with appropriate growth factors, to allow the differentiation of precursor cells into large numbers of macrophages. This procedure is easy and inexpensive except for the use of M-CSF, the macrophage colony stimulating factor, and it is characterised by high yield and reproducibility. Once obtained, bone marrow-derived macrophages (BMMs) can be used for a considerable number of functional and structural assays and are commonly regarded as a model for the role of resident macrophages in the innate immune system.
Keyword Bone marrow
Macrophage colony stimulating factor
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Book Chapter
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School of Biomedical Sciences Publications
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Created: Wed, 23 May 2012, 19:30:22 EST by Bacsweet Kaur on behalf of School of Biomedical Sciences