Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25:H4) and bla CTX-M-15among extended-spectrum-β- lactamase-producing E. coli from the United States, 2000 to 2009

Johnson, James R., Urban, Carl, Weissman, Scott J., Jorgensen, James H., Lewis, James S., Hansen, Glen, Edelstein, Paul H., Robicsek, Ari, Cleary, Timothy, Adachi, Javier, Paterson, David, Quinn, John, Hanson, Nancy D., Johnston, Brian D., Clabots, Connie, Kuskowski, Michael A. and AMERECUS Investigators (2012) Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25:H4) and bla CTX-M-15among extended-spectrum-β- lactamase-producing E. coli from the United States, 2000 to 2009. Antimicrobial Agents and Chemotherapy, 56 5: 2364-2370. doi:10.1128/AAC.05824-11

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Author Johnson, James R.
Urban, Carl
Weissman, Scott J.
Jorgensen, James H.
Lewis, James S.
Hansen, Glen
Edelstein, Paul H.
Robicsek, Ari
Cleary, Timothy
Adachi, Javier
Paterson, David
Quinn, John
Hanson, Nancy D.
Johnston, Brian D.
Clabots, Connie
Kuskowski, Michael A.
AMERECUS Investigators
Total Author Count Override 23
Title Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25:H4) and bla CTX-M-15among extended-spectrum-β- lactamase-producing E. coli from the United States, 2000 to 2009
Formatted title
Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25:H4) and blaCTX-M-15 among extended-spectrum-β- lactamase-producing E. coli from the United States, 2000 to 2009
Journal name Antimicrobial Agents and Chemotherapy   Check publisher's open access policy
ISSN 0066-4804
1098-6596
Publication date 2012-05-01
Sub-type Article (original research)
DOI 10.1128/AAC.05824-11
Open Access Status File (Publisher version)
Volume 56
Issue 5
Start page 2364
End page 2370
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2013
Language eng
Formatted abstract
Escherichia coli sequence type ST131 (from phylogenetic group B2), often carrying the extended-spectrum-β-lactamase (ESBL) gene bla CTX-M-15, is an emerging globally disseminated pathogen that has received comparatively little attention in the United States. Accordingly, a convenience sample of 351 ESBL-producing E. coli isolates from 15 U.S. centers (collected in 2000 to 2009) underwent PCR-based phylotyping and detection of ST131 and bla CTX-M-15. A total of 200 isolates, comprising 4 groups of 50 isolates each that were (i) bla CTX-M-15 negative non-ST131, (ii) bla CTX-M-15 positive non-ST131, (iii) bla CTX-M-15 negative ST131, or (iv) bla CTX-M-15 positive ST131, also underwent virulence genotyping, antimicrobial susceptibility testing, and pulsed-field gel electrophoresis (PFGE). Overall, 201 (57%) isolates exhibited bla CTX-M-15, whereas 165 (47%) were ST131. ST131 accounted for 56% of bla CTX-M-15-positive-versus 35% of bla CTX-M-15-negative isolates (P<0.001). Whereas ST131 accounted for 94% of the 175 total group B2 isolates, non- ST131 isolates were phylogenetically distributed by bla CTX-M-15 status, with groups A (bla CTX-M-15-positive isolates) andD(bla CTX-M-15- negative isolates) predominating. Both bla CTX-M-15 and ST131 occurred at all participating centers, were recovered from children and adults, increased significantly in prevalence post-2003, and were associated with molecularly inferred virulence. Compared with non- ST131 isolates, ST131 isolates had higher virulence scores, distinctive virulence profiles, and more-homogeneous PFGE profiles. bla CTX-M-15 was associated with extensive antimicrobial resistance and ST131 with fluoroquinolone resistance. Thus, E. coli ST131 and bla CTX-M-15 are emergent, widely distributed, and predominant among ESBL-positive E. coli strains in the United States, among children and adults alike. Enhanced virulence and antimicrobial resistance have likely promoted the epidemiological success of these emerging public health threats.
Keyword Field gel-electrophoresis
Emergence
Infections
Strains
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2013 Collection
School of Medicine Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 61 times in Thomson Reuters Web of Science Article | Citations
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