Identification of a fructose-1,6-bisphosphate aldolase gene and association of the single nucleotide polymorphisms with growth traits in the clam Meretrix meretrix

Wang, Chao, Wang, Hongxia, Li, Yan and Liu, Baozhong (2012) Identification of a fructose-1,6-bisphosphate aldolase gene and association of the single nucleotide polymorphisms with growth traits in the clam Meretrix meretrix. Molecular Biology Reports, 39 4: 5017-5024. doi:10.1007/s11033-011-1298-9


Author Wang, Chao
Wang, Hongxia
Li, Yan
Liu, Baozhong
Title Identification of a fructose-1,6-bisphosphate aldolase gene and association of the single nucleotide polymorphisms with growth traits in the clam Meretrix meretrix
Formatted title
Identification of a fructose-1,6-bisphosphate aldolase gene and association of the single nucleotide polymorphisms with growth traits in the clam Meretrix meretrix
Journal name Molecular Biology Reports   Check publisher's open access policy
ISSN 0301-4851
1573-4978
Publication date 2012-04-01
Year available 2011
Sub-type Article (original research)
DOI 10.1007/s11033-011-1298-9
Volume 39
Issue 4
Start page 5017
End page 5024
Total pages 8
Place of publication Dordrecht, Netherlands
Publisher Springer
Collection year 2012
Language eng
Formatted abstract
This study investigated whether there were single nucleotide polymorphisms (SNPs) in fructose-1,6-bisphosphate aldolase (FBA) gene associated with growth traits of the clam Meretrix meretrix. A FBA gene was identified in M. meretrix and its deduced amino acid residues shared high identity with type I aldolase. The FBA (MmeFBA) mRNA expression profile was examined by real-time PCR in different tissues and the significantly high expression level in foot and adduct muscle suggests that MmeFBA is a muscle type aldolase which functions in glycolytic pathway. In the MmeFBA gene, we identified four intron SNPs and three exon SNPs including a nonsynonymous SNP (mmfbae-2). These SNPs were genotyped in 205 clams from two clam populations with significantly different growth performance. Results showed that allele frequencies of three SNPs (mmfbai-1, mmfbai-3 and mmfbae-2) and the genotype frequency of mmfbai-1 were all significantly different between the two populations. The haplotype analysis further supported the three SNPs distributed differently between the two populations. This study successively characterized three growth-related SNPs in a gene involved in energy metabolism of M. meretrix. These findings could contribute the development of phenotype-selective breeding program in M. meretrix.
Keyword Meretrix meretrix
SNP
FBA gene
Growth trait
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 8 December 2011.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Biological Sciences Publications
 
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Created: Tue, 08 May 2012, 20:52:38 EST by Yan Li on behalf of School of Agriculture and Food Sciences