G gamma 1+G gamma 2+G gamma 3=G beta: The search for heterotrimeric G-protein gamma subunits in Arabidopsis is over

Thung, Leena, Trusov, Yuri, Chakravorty, David and Botella, Jose Ramon (2012) G gamma 1+G gamma 2+G gamma 3=G beta: The search for heterotrimeric G-protein gamma subunits in Arabidopsis is over. Journal of Plant Physiology, 169 5: 542-545. doi:10.1016/j.jplph.2011.11.010


Author Thung, Leena
Trusov, Yuri
Chakravorty, David
Botella, Jose Ramon
Title G gamma 1+G gamma 2+G gamma 3=G beta: The search for heterotrimeric G-protein gamma subunits in Arabidopsis is over
Formatted title
Gγ1+Gγ2+Gγ3=Gβ: The search for heterotrimeric G-protein γ subunits in Arabidopsis is over
Journal name Journal of Plant Physiology   Check publisher's open access policy
ISSN 0176-1617
1618-1328
Publication date 2012-03-15
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.jplph.2011.11.010
Open Access Status Not yet assessed
Volume 169
Issue 5
Start page 542
End page 545
Total pages 4
Place of publication Jena, Germany
Publisher Urban und Fischer
Language eng
Subject 1110 Plant Science
1314 Physiology
1102 Agronomy and Crop Science
Abstract In Arabidopsis, heterotrimeric G-proteins consist of one Get (GPA1), one G beta (AGB1) and three G gamma (AGG1, AGG2 and AGG3) subunits. G beta and G gamma subunits function as obligate heterodimers, therefore any phenotypes observed in G beta-deficient mutants should be apparent in Goy-deficient mutants. Nevertheless, the first two G gamma subunits discovered failed to explain many of the phenotypes shown by the agb1 mutants in Arabidopsis, prompting the search for additional G gamma subunits. The recent discovery of an additional, although quite atypical, G gamma subunit in Arabidopsis (AGG3) has helped to complete the picture and explains almost all of the missing agb1 'orphan' phenotypes. There is nevertheless still one unexplained phenotype, the reduction in rosette size reported for agb1, that has not been observed in any of the individual agg mutants or the double agg1 agg2 mutant. We have now created a triple gamma mutant (agg1agg2agg3) in Arabidopsis and show that it recapitulates the remaining 'orphan' agb1 phenotypes. Triple agg1agg2agg3 mutants show the reduction in rosette size previously observed in agb1 mutants. In addition we show that small differences in flower and silique size observed between agb1 and agg3 mutants are also accounted for by the triple agg1agg2agg3 mutant. Our results strongly suggest that there are no additional members of the G-protein family remaining to be discovered in Arabidopsis. (C) 2011 Elsevier GmbH. All rights reserved.
Formatted abstract
In Arabidopsis, heterotrimeric G-proteins consist of one Gα (GPA1), one Gβ (AGB1) and three Gγ (AGG1, AGG2 and AGG3) subunits. Gβ and Gγ subunits function as obligate heterodimers, therefore any phenotypes observed in Gβ-deficient mutants should be apparent in Gγ-deficient mutants. Nevertheless, the first two Gγ subunits discovered failed to explain many of the phenotypes shown by the agb1 mutants in Arabidopsis, prompting the search for additional Gγ subunits. The recent discovery of an additional, although quite atypical, Gγ subunit in Arabidopsis (AGG3) has helped to complete the picture and explains almost all of the missing agb1 ‘orphan’ phenotypes. There is nevertheless still one unexplained phenotype, the reduction in rosette size reported for agb1, that has not been observed in any of the individual agg mutants or the double agg1agg2 mutant. We have now created a triple gamma mutant (agg1agg2agg3) in Arabidopsis and show that it recapitulates the remaining ‘orphan’ agb1 phenotypes. Triple agg1agg2agg3 mutants show the reduction in rosette size previously observed in agb1 mutants. In addition we show that small differences in flower and silique size observed between agb1 and agg3 mutants are also accounted for by the triple agg1agg2agg3 mutant. Our results strongly suggest that there are no additional members of the G-protein family remaining to be discovered in Arabidopsis.
Keyword Arabidopsis
Cell signaling
G-protein beta gamma subunit
Heterotrimeric G-protein
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 28 December 2011. Published under Short Communications.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Agriculture and Food Sciences
 
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