Co-circulation of four human Coronaviruses (HCoVs) in Queensland children with acute respiratory tract illnesses in 2004

Mackay, Ian M., Arden, Katherine E., Speicher, David J., O'Neil, Nicholas T., McErlean, Peter K., Greer, Ristan M., Nissen, Michael D. and Sloots, Theo P. (2012) Co-circulation of four human Coronaviruses (HCoVs) in Queensland children with acute respiratory tract illnesses in 2004. Viruses, 4 4: 637-653. doi:10.3390/v4040637

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads

Author Mackay, Ian M.
Arden, Katherine E.
Speicher, David J.
O'Neil, Nicholas T.
McErlean, Peter K.
Greer, Ristan M.
Nissen, Michael D.
Sloots, Theo P.
Title Co-circulation of four human Coronaviruses (HCoVs) in Queensland children with acute respiratory tract illnesses in 2004
Journal name Viruses   Check publisher's open access policy
ISSN 1999-4915
Publication date 2012-04-23
Year available 2012
Sub-type Article (original research)
DOI 10.3390/v4040637
Open Access Status DOI
Volume 4
Issue 4
Start page 637
End page 653
Total pages 17
Place of publication Basel, Switzerland
Publisher M D P I AG
Language eng
Subject 2725 Infectious Diseases
2406 Virology
Abstract Acute respiratory illnesses (ARIs) with unconfirmed infectious aetiologies peak at different times of the year. Molecular diagnostic assays reduce the number of unconfirmed ARIs compared to serology- or culture-based techniques. Screening of 888 inpatient and outpatient respiratory specimens spanning late autumn through to early spring, 2004, identified the presence of a human coronavirus (HCoV) on 74 occasions (8.3% of all specimens and 26.3% of all respiratory virus detections). Prevalence peaked in August (late winter in the southern hemisphere) when they were detected in 21.9% of specimens tested. HCoV-HKU1 and HCoV-OC43 comprised 82.4% of all HCoVs detected. Positive specimens were used to develop novel reverse transcriptase real-time PCRs (RT-rtPCRs) for HCoV detection. An objective clinical severity score was assigned to each positive HCoV patient. Severity scores were similar to those from a random selection of young children who were positive for respiratory syncytial virus at a different time but from the same specimen population. During the cooler months of 2004, sensitive and specific RT-rtPCRs identified the concurrent circulation of all four HCoVs, a quarter of which co-occurred with another virus and most of which were from children under the age of two years.
Keyword Respiratory virus
Coronavirus
HCoV-HKU1
HCoV-NL63
HCoV-229E
HCoV-OC43
Real-time PCR
Clinical impact
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 10281
50028
HREC/06/QRCH/97
2008004496
Institutional Status UQ

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 14 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 27 Apr 2012, 19:16:05 EST by Lucy O'Brien on behalf of School of Chemistry & Molecular Biosciences