Identification of naive or antigen-experienced human CD8(+) T cells by expression of costimulation and chemokine receptors: Analysis of the human cytomegalovirus-specific CD8(+) T cell response

Wills, MR, Okecha, G, Weekes, MP, Gandhi, MK, Sissons, PJG and Carmichael, AJ (2002) Identification of naive or antigen-experienced human CD8(+) T cells by expression of costimulation and chemokine receptors: Analysis of the human cytomegalovirus-specific CD8(+) T cell response. Journal of Immunology, 168 11: 5455-5464. doi:10.1038/icb.2015.17


Author Wills, MR
Okecha, G
Weekes, MP
Gandhi, MK
Sissons, PJG
Carmichael, AJ
Title Identification of naive or antigen-experienced human CD8(+) T cells by expression of costimulation and chemokine receptors: Analysis of the human cytomegalovirus-specific CD8(+) T cell response
Journal name Journal of Immunology   Check publisher's open access policy
ISSN 0022-1767
Publication date 2002-06-01
Year available 2002
Sub-type Article (original research)
DOI 10.1038/icb.2015.17
Open Access Status DOI
Volume 168
Issue 11
Start page 5455
End page 5464
Total pages 10
Publisher AMER ASSOC IMMUNOLOGISTS
Language eng
Abstract Basic parameters of the naive antigen (Ag)-specific T-cell repertoire in humans remain poorly defined. Systematic characterization of this 'ground state' immunity in comparison with memory will allow a better understanding of clonal selection during immune challenge. Here, we used high-definition cell isolation from umbilical cord blood samples to establish the baseline frequency, phenotype and T-cell antigen receptor (TCR) repertoire of CD8(+) T-cell precursor populations specific for a range of viral and self-derived Ags. Across the board, these precursor populations were phenotypically naive and occurred with hierarchical frequencies clustered by Ag specificity. The corresponding patterns of TCR architecture were highly ordered and displayed partial overlap with adult memory, indicating biased structuring of the T-cell repertoire during Ag-driven selection. Collectively, these results provide new insights into the complex nature and dynamics of the naive T-cell compartment.
Keyword Lymphocyte Ctl Response
In-Vivo
Melanoma Patients
Memory Cells
Subsets
Clones
Visualization
Uchl1
Cd27
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 100326
APP613664
BB/H001085/1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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