Delivery of dermatan sulfate from polyelectrolyte complex-containing alginate composite microspheres for tissue regeneration

Wen, Yanhong, Grøndahl, Lisbeth, Gallego, Monica R., Jorgensen, Lene, Møller, Eva H. and Nielsen, Hanne M. (2012) Delivery of dermatan sulfate from polyelectrolyte complex-containing alginate composite microspheres for tissue regeneration. Biomacromolecules, 13 3: 905-917. doi:10.1021/bm201821x


Author Wen, Yanhong
Grøndahl, Lisbeth
Gallego, Monica R.
Jorgensen, Lene
Møller, Eva H.
Nielsen, Hanne M.
Title Delivery of dermatan sulfate from polyelectrolyte complex-containing alginate composite microspheres for tissue regeneration
Journal name Biomacromolecules   Check publisher's open access policy
ISSN 1525-7797
1526-4602
Publication date 2012-03-12
Sub-type Article (original research)
DOI 10.1021/bm201821x
Volume 13
Issue 3
Start page 905
End page 917
Total pages 13
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Abstract Dermatan sulfate (DS) is a glycosaminoglycan (GAG) with a great potential as a new therapeutic agent in tissue engineering. The aim of the present study was to investigate the formation of polyelectrolyte complexes (PECs) between chitosan and dermatan sulfate (CS/DS) and delivery of DS from PEC-containing alginate/chitosan/dermatan sulfate (Alg/CS/DS) microspheres for application in tissue regeneration. The CS/DS complexes were initially formed at different conditions including varying CS/DS ratio (positive/negative charge ratio), buffer, and pH. The obtained CS/DS complexes exhibited stronger electrostatic interaction, smaller complex size, and more stable colloidal structure when chitosan was in large excess (CS/DS 3:1) and prepared at pH 3.5 as compared to pH 5 using acetate buffer. The CS/DS complexes were subsequently incorporated into an alginate matrix by spray drying to form Alg/CS/DS composite microspheres with a DS encapsulation efficiency of 90–95%. The excessive CS induced a higher level of sustained DS release into Tris buffer (pH 7.4) from the microspheres formulated at pH 3.5; however, the amount of CS did not have a significant effect on the release from the microspheres formulated at pH 5. Significant cell proliferation was stimulated by the DS released from the microspheres in vitro. The present results provide a promising drug delivery strategy using PECs for sustained release of DS from microspheres intended for site-specific drug delivery and ultimately for use in tissue engineering.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Mon, 02 Apr 2012, 20:29:56 EST by Lucy O'Brien on behalf of School of Chemistry & Molecular Biosciences