Mammalian cells as biopharmaceutical production hosts in the age of omics

Dietmair, Stefanie, Nielsen, Lars K. and Timmins, Nicholas E. (2012) Mammalian cells as biopharmaceutical production hosts in the age of omics. Biotechnology Journal, 7 1: 75-89. doi:10.1002/biot.201100369


Author Dietmair, Stefanie
Nielsen, Lars K.
Timmins, Nicholas E.
Title Mammalian cells as biopharmaceutical production hosts in the age of omics
Journal name Biotechnology Journal   Check publisher's open access policy
ISSN 1860-6768
1860-7314
Publication date 2012-01-01
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1002/biot.201100369
Volume 7
Issue 1
Start page 75
End page 89
Total pages 15
Place of publication Weinheim, Germany
Publisher Wiley
Collection year 2013
Language eng
Abstract Mammalian cells are important hosts for the production of a wide range of biopharmaceuticals due to their ability to produce correctly folded and glycosylated proteins. Compared to microbes and yeast, however, the productivity of mammalian cells is low because of their comparatively slow growth rate, tendency to undergo apoptosis, and low production capacities. While much effort has been invested in the engineering of mammalian cells with superior production characteristics, the success of these approaches has been limited to date. One factor responsible for this lack of success is our limited understanding of the cellular basis for high productivity, and of how discrete mechanisms within a cell contribute to the overall phenotype. Aiming to measure and characterize all cellular components at different functional levels, omics technologies have the potential to improve our understanding of mammalian cell physiology, elucidating new targets for the generation of a superior host cell line. This review provides a comprehensive analysis of recent examples of omics studies in the context of mammalian cells as production hosts, highlighting both the challenges and successes in the application of these powerful technologies.
Keyword Biopharmaceuticals
Mammalian cell
Omics
Protein production
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 16 December 2011.

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2013 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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