Confirming the revised C-terminal domain of the MscL crystal structure

Maurer, Joshua A., Elmore, Donald E., Clayton, Daniel, Xiong, Li, Lester, Henry A. and Dougherty, Dennis A. (2008) Confirming the revised C-terminal domain of the MscL crystal structure. Biophysical Journal, 94 12: 4662-4667. doi:10.1529/biophysj.107.127365


Author Maurer, Joshua A.
Elmore, Donald E.
Clayton, Daniel
Xiong, Li
Lester, Henry A.
Dougherty, Dennis A.
Title Confirming the revised C-terminal domain of the MscL crystal structure
Journal name Biophysical Journal   Check publisher's open access policy
ISSN 0006-3495
1542-0086
Publication date 2008-06-15
Sub-type Article (original research)
DOI 10.1529/biophysj.107.127365
Open Access Status DOI
Volume 94
Issue 12
Start page 4662
End page 4667
Total pages 6
Place of publication St. Louis, MO, United States
Publisher Cell Press
Language eng
Formatted abstract
The structure of the C-terminal domain of the mechanosensitive channel of large conductance (MscL) has generated significant controversy. As a result, several structures have been proposed for this region: the original crystal structure (1MSL) of the Mycobacterium tuberculosis homolog (Tb), a model of the Escherichia coli homolog, and, most recently, a revised crystal structure of Tb-MscL (2OAR). To understand which of these structures represents a physiological conformation, we measured the impact of mutations to the C-terminal domain on the thermal stability of Tb-MscL using circular dichroism and performed molecular dynamics simulations of the original and the revised crystal structures of Tb-MscL. Our results imply that this region is helical and adopts an α-helical bundle conformation similar to that observed in the E. coli MscL model and the revised Tb-MscL crystal structure.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Institute for Molecular Bioscience - Publications
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Wed, 21 Mar 2012, 00:39:32 EST by Susan Allen on behalf of Institute for Molecular Bioscience