Novel venom gene discovery in the platypus

Whittington, Camilla M., Papenfuss, Anthony T., Locke, Devin P., Mardis, Elaine R., Wilson, Richard K., Abubucker, Sahar, Mitreva, Makedonka, Wong, Emily S. W., Hsu, Arthur L., Kuchel, Philip W., Belov, Katherine and Warren, Wesley C. (2010) Novel venom gene discovery in the platypus. Genome Biology, 11 9: R95.1-R95.13. doi:10.1186/gb-2010-11-9-r95


Author Whittington, Camilla M.
Papenfuss, Anthony T.
Locke, Devin P.
Mardis, Elaine R.
Wilson, Richard K.
Abubucker, Sahar
Mitreva, Makedonka
Wong, Emily S. W.
Hsu, Arthur L.
Kuchel, Philip W.
Belov, Katherine
Warren, Wesley C.
Title Novel venom gene discovery in the platypus
Journal name Genome Biology   Check publisher's open access policy
ISSN 1474-7596
1474-760X
Publication date 2010-09-29
Sub-type Article (original research)
DOI 10.1186/gb-2010-11-9-r95
Open Access Status DOI
Volume 11
Issue 9
Start page R95.1
End page R95.13
Total pages 13
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background
To date, few peptides in the complex mixture of platypus venom have been identified and sequenced, in part due to the limited amounts of platypus venom available to study. We have constructed and sequenced a cDNA library from an active platypus venom gland to identify the remaining components.

Results

We identified 83 novel putative platypus venom genes from 13 toxin families, which are homologous to known toxins from a wide range of vertebrates (fish, reptiles, insectivores) and invertebrates (spiders, sea anemones, starfish). A number of these are expressed in tissues other than the venom gland, and at least three of these families (those with homology to toxins from distant invertebrates) may play non-toxin roles. Thus, further functional testing is required to confirm venom activity. However, the presence of similar putative toxins in such widely divergent species provides further evidence for the hypothesis that there are certain protein families that are selected preferentially during evolution to become venom peptides. We have also used homology with known proteins to speculate on the contributions of each venom component to the symptoms of platypus envenomation.

Conclusions
This study represents a step towards fully characterizing the first mammal venom transcriptome. We have found similarities between putative platypus toxins and those of a number of unrelated species, providing insight into the evolution of mammalian venom.
Keyword Ornithorhynchus-anatinus venom
Stonefish synanceia-verrucosa
Shrew blarina-brevicauda
Factor pathway inhibitor
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
Institute for Molecular Bioscience - Publications
 
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Created: Wed, 14 Mar 2012, 01:38:47 EST by Susan Allen on behalf of Institute for Molecular Bioscience