Novel roles of the chemorepellent axon guidance molecule RGMa in cell migration and adhesion

Lah, Grace J. and Key, Brian (2012) Novel roles of the chemorepellent axon guidance molecule RGMa in cell migration and adhesion. Molecular and Cellular Biology, 32 5: 968-980. doi:10.1128/MCB.06128-11

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Author Lah, Grace J.
Key, Brian
Title Novel roles of the chemorepellent axon guidance molecule RGMa in cell migration and adhesion
Journal name Molecular and Cellular Biology   Check publisher's open access policy
ISSN 0270-7306
Publication date 2012-03-01
Sub-type Article (original research)
DOI 10.1128/MCB.06128-11
Open Access Status File (Publisher version)
Volume 32
Issue 5
Start page 968
End page 980
Total pages 13
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
The repulsive guidance molecule A (RGMa) is a contact-mediated axon guidance molecule that has significant roles in central nervous system (CNS) development. Here we have examined whether RGMa has novel roles in cell migration and cell adhesion outside the nervous system. RGMa was found to stimulate cell migration from Xenopus animal cap explants in a neogenin-dependent and BMP-independent manner. RGMa also stimulated the adhesion of Xenopus animal cap cells, and this adhesion was dependent on neogenin and independent of calcium. To begin to functionally characterize the role of specific domains in RGMa, we assessed the migratory and adhesive activities of deletion mutants. RGMa lacking the partial von Willebrand factor type D (vWF) domain preferentially perturbed cell adhesion, while mutants lacking the RGD motif affected cell migration. We also revealed that manipulating the levels of RGMa in vivo caused major migration defects during Xenopus gastrulation. We have revealed here novel roles of RGMa in cell migration and adhesion and demonstrated that perturbations to the homeostasis of RGMa expression can severely disrupt major morphogenetic events. These results have implications for understanding the role of RGMa in both health and disease.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
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