Quantitative PCR-based genome size estimation of the astigmatid mites Sarcoptes scabiei, Psoroptes ovis and Dermatophagoides pteronyssinus

Mounsey, Kate E., Willis, Charlene, Burgess, Stewart T.G., Holt, Deborah C., McCarthy, James and Fischer, Katja (2012) Quantitative PCR-based genome size estimation of the astigmatid mites Sarcoptes scabiei, Psoroptes ovis and Dermatophagoides pteronyssinus. Parasites and Vectors, 5 1: 3-1-3-7. doi:10.1186/1756-3305-5-3


Author Mounsey, Kate E.
Willis, Charlene
Burgess, Stewart T.G.
Holt, Deborah C.
McCarthy, James
Fischer, Katja
Title Quantitative PCR-based genome size estimation of the astigmatid mites Sarcoptes scabiei, Psoroptes ovis and Dermatophagoides pteronyssinus
Journal name Parasites and Vectors   Check publisher's open access policy
ISSN 1756-3305
Publication date 2012-01-01
Sub-type Article (original research)
DOI 10.1186/1756-3305-5-3
Open Access Status DOI
Volume 5
Issue 1
Start page 3-1
End page 3-7
Total pages 7
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background:
The lack of genomic data available for mites limits our understanding of their biology. Evolving high-throughput sequencing technologies promise to deliver rapid advances in this area, however, estimates of genome size are initially required to ensure sufficient coverage.

Methods.:
Quantitative real-time PCR was used to estimate the genome sizes of the burrowing ectoparasitic mite Sarcoptes scabiei, the non-burrowing ectoparasitic mite Psoroptes ovis, and the free-living house dust mite Dermatophagoides pteronyssinus. Additionally, the chromosome number of S. scabiei was determined by chromosomal spreads of embryonic cells derived from single eggs.

Results:
S. scabiei cells were shown to contain 17 or 18 small (< 2 M) chromosomes, suggesting an XO sex-determination mechanism. The average estimated genome sizes of S. scabiei and P. ovis were 96 ( 7) Mb and 86 ( 2) Mb respectively, among the smallest arthropod genomes reported to date. The D. pteronyssinus genome was estimated to be larger than its parasitic counterparts, at 151 Mb in female mites and 218 Mb in male mites.

Conclusions:
This data provides a starting point for understanding the genetic organisation and evolution of these astigmatid mites, informing future sequencing projects. A comparitive genomic approach including these three closely related mites is likely to reveal key insights on mite biology, parasitic adaptations and immune evasion.
Keyword Sarcoptes scabiei
Psoroptes ovis
Dermatophagoides pteronyssinus
Genome size
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published: 4 January 2012.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Medicine Publications
 
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