Single fly tethered paradigms

van Swinderen, Bruno (2011) Single fly tethered paradigms. Cold Spring Harbor Protocol, 2011 12: 1488-1491. doi:10.1101/pdb.prot066910


Author van Swinderen, Bruno
Title Single fly tethered paradigms
Language of Title eng
Journal name Cold Spring Harbor Protocol   Check publisher's open access policy
Language of Journal Name eng
ISSN 1940-3402
1559-6095
Publication date 2011-12-01
Sub-type Other
DOI 10.1101/pdb.prot066910
Volume 2011
Issue 12
Start page 1488
End page 1491
Total pages 4
Place of publication Woodbury, NY, U.S.A.
Publisher Cold Spring Harbor Laboratory Press
Language eng
Formatted abstract
The most successful approaches for studying visual perception and visual learning in Drosophila have been single fly paradigms in which tethered individuals respond to different visual stimuli, as described here. The equipment and protocols involved are quite sophisticated and differ depending on whether behavior or electrophysiology will be pursued. For either approach, flies must first be secured to a metal wire. This is typically performed by first cooling flies down to 4°C and then gluing them to a copper or tungsten wire with ultraviolet (UV)-activated cement. For electrophysiology, tethering requires a few extra steps to accommodate the placement of electrodes. Prepared individuals are then placed inside a cylindrical arena where images can be presented, or in front of a computer screen or even in front of a laptop. Flight dynamics or brain activity in response to visual stimuli is recorded by using a variety of specialized and/or commercially available electronic devices.
Q-Index Code CX
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Adapted from Drosophila Neurobiology (ed. Zhang et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2010.

Document type: Journal Article
Sub-type: Other
Collections: Non HERDC
Queensland Brain Institute Publications
 
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Created: Tue, 13 Mar 2012, 02:24:48 EST by Debra McMurtrie on behalf of Queensland Brain Institute