Characterization of germ cells from pre-pubertal bull calves in preparation for germ cell transplantation

Herrid, Muren, Davey, Rhonda J. and Hill, Jonathan R. (2007) Characterization of germ cells from pre-pubertal bull calves in preparation for germ cell transplantation. Cell and Tissue Research, 330 2: 321-329. doi:10.1007/s00441-007-0445-z


Author Herrid, Muren
Davey, Rhonda J.
Hill, Jonathan R.
Title Characterization of germ cells from pre-pubertal bull calves in preparation for germ cell transplantation
Journal name Cell and Tissue Research   Check publisher's open access policy
ISSN 0302-766X
1432-0878
Publication date 2007-11-01
Sub-type Article (original research)
DOI 10.1007/s00441-007-0445-z
Open Access Status Not yet assessed
Volume 330
Issue 2
Start page 321
End page 329
Total pages 9
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract Although methods to assess testis cell populations are established in mice, the detailed validation of similar methods for bovine testis cells is necessary for the development of emerging technologies such as male germ cell transplantation. As young calves provide donor cells for germ cell transplantation, we characterized cell populations from three key pre-pubertal stages. Nine Angus bull calves were selected to represent three stages of testis development at ages (and testis weights) of 2-3 months (Stage 1, 10 g), 4-5 months (Stage 2, 35 g), and 6-7 months (Stage 3, 70 g). The proportion and absolute numbers of germ and somatic cells in fixed sections and from enzymatically dissociated seminiferous tubules were assessed. Germ cells were identified by DBA and PGP9.5 staining, and Sertoli cells by vimentin and GATA-4 staining. Staining of serial sections confirmed that DBA and PGP9.5 identified similar cells, which were complementary to those stained for vimentin and GATA-4. In fixed tubules, the proportion of cells within tubules that were positive for DBA and PGP9.5 increased nearly three-fold from Stage 1 to Stage 2 with no further increase at Stage 3. Absolute numbers of spermatogonia also increased between Stages 1 and 2. After enzymatic dissociation of tubules, three times more DBA- and PGP9.5-positive cells were isolated from Stage 3 testes than from either Stage 1 or 2 testes. A higher proportion of spermatogonia was observed after enzymatic isolation than were present in seminiferous tubules. These data should help to predict the yield and expected proportions of spermatogonia from three distinct stages of testis development in pre-pubertal bull calves.
Keyword Spermatogonia
Stem cell
Isolation
Transplantation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 39 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 40 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 09 Mar 2012, 04:52:20 EST by Professor Jonathan Hill on behalf of School of Veterinary Science