TNF-alpha promotes IFN-gamma-induced CD40 expression and antigen process in Myb-transformed hematological cells

Gu, Wenyi, Chen, Jeizhong, Yang, Lei and Zhao, Kong-Nan (2012) TNF-alpha promotes IFN-gamma-induced CD40 expression and antigen process in Myb-transformed hematological cells. The Scientific World Journal, 2012 621969.1-621969.11. doi:10.1100/2012/621969

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Author Gu, Wenyi
Chen, Jeizhong
Yang, Lei
Zhao, Kong-Nan
Title TNF-alpha promotes IFN-gamma-induced CD40 expression and antigen process in Myb-transformed hematological cells
Formatted title
TNF-α promotes IFN-γ-induced CD40 expression and antigen process in Myb-transformed hematological cells
Journal name The Scientific World Journal   Check publisher's open access policy
ISSN 1537-744X
Publication date 2012-01-01
Sub-type Article (original research)
DOI 10.1100/2012/621969
Open Access Status DOI
Volume 2012
Start page 621969.1
End page 621969.11
Total pages 11
Editor Fulvio D'Acquisto
Place of publication New York, NY, U.S.A.
Publisher The ScientificWorld
Language eng
Subject 1300 Biochemistry, Genetics and Molecular Biology
2300 Environmental Science
Abstract Tumour necrosis factor-α, interferon-γ and interleukin-4 are critical cytokines in regulating the immune responses against infections and tumours. In this study, we investigated the effects of three cytokines on CD40 expression in Myb-transformed hematological cells and their regulatory roles in promoting these cells into dendritic cells. We observed that both interleukin-4 and interferon-γ increased CD40 expression in these hematological cells in a dose-dependent manner, although the concentration required for interleukin-4 was significantly higher than that for interferon-γ. We found that tumour necrosis factor-α promoted CD40 expression induced by interferon-γ, but not by interleukin-4. Our data showed that tumour necrosis factor-α plus interferon - treated Myb-transformed hematological cells had the greatest ability to take up and process the model antigen DQ-Ovalbumin. Tumour necrosis factor-α also increased the ability of interferon-γ to produce the mixed lymphocyte reaction to allogenic T cells. Furthermore, only cotreatment with tumour necrosis factor-α and interferon-γ induced Myb-transformed hematological cells to express interleukin-6. These results suggest that tumour necrosis factor-α plays a key regulatory role in the development of dendritic cells from hematological progenitor cells induced by interferon-γ. Copyright 2012 Wenyi Gu et al.
Formatted abstract
Tumour-necrosis factor-α (TNF-α) is a pro-inflammatory cytokine that is involved in the immunity against infections and tumours and interferon-γ (IFN-γ) and interleukin-4 (IL-4) are two critical cytokines in regulating the development of immune cells and the immune response. In this study, we investigated the effect of the three cytokines on CD40 expression in Myb-transformed hematological cells (MTHCs) and their regulatory roles in the development of MTHCs into either macrophages or dendritic-like cells (DCs). We observed that both IL-4 and IFN-γ could increase CD40 expression in MTHCs in a dose-dependent manner, although the concentration required for IL-4 was significantly higher than that for IFN-γ. We found that TNF-α could significantly promote CD40 expression induced by IFN-g, but not by IL-4 in MTHCs. Our data showed that TNF-α plus IFN-γ-treated MTHCs had the greatest ability to take up and process the model antigen DQ-Ovalbumin. TNF-α also increased the ability of IFN-γ to produce the mixed lymphocyte reaction to allogenic T cells. Furthermore, only co-treatment with TNF-α and IFN-g could induce MTHCs to express IL-6; and TNF-α could reduce the production of IL-12 induced by IFN-g. These results suggest that TNF-α plays a key regulatory role in the development of dendritic-like cells from hematological progenitor cells induced by IFN-g and their immunological function.

Synopsis: TNF-α significantly increases IFN-g induced CD40 expression in MTHCs and thus promotes DC development. TNF-α plus IFN-γ-treated MTHCs had the greatest ability to take up and process model antigen DQ-Ovalbumin.
Keyword MTHCs
TNF-α
IFN-γ
IL-4
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article # 621969. Authors prepress title: "TNF-α synergizes IFN-γ-induced CD40 expression and antigen processing in Myb-transformed haematological cells".

 
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Created: Tue, 06 Mar 2012, 22:04:05 EST by Roheen Gill on behalf of Aust Institute for Bioengineering & Nanotechnology