Human labour is associated with decreased cytoplasmic FoxO4

Lim, R., Riley, C., Barker, G., Rice, G. E. and Lappas, M. (2012) Human labour is associated with decreased cytoplasmic FoxO4. Placenta, 33 1: 52-59. doi:10.1016/j.placenta.2011.10.004

Author Lim, R.
Riley, C.
Barker, G.
Rice, G. E.
Lappas, M.
Title Human labour is associated with decreased cytoplasmic FoxO4
Journal name Placenta   Check publisher's open access policy
ISSN 0143-4004
Publication date 2012-01-01
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.placenta.2011.10.004
Open Access Status Not Open Access
Volume 33
Issue 1
Start page 52
End page 59
Total pages 8
Place of publication Oxford, United Kingdom
Publisher Elsevier
Language eng
Formatted abstract
Forkhead box O (FoxO) proteins function primarily as transcription factors in the nucleus where they bind to their cognate DNA targeting sequences. FoxO regulated genes include those involved in cellular stress responses, inflammation and apoptosis; all of which are involved in the processes of human labour and delivery. We have previously identified Forkhead box O4 (FoxO4) proteins in human gestational tissues; there is, however, no data is available on the role of FoxO4 in the processes of human labour and delivery. Thus the aim of this study was to determine the effect of (i) human labour, preterm chorioamnionitis and pro-inflammatory stimuli on the expression of FoxO4 in human placenta and fetal membranes; and (ii) FoxO4 knockdown by siRNA on the expression of pro-labour mediators. Quantitative RT-PCR (qRT-PCR), immunohistochemistry and/or Western blotting was used to analyse the expression of FoxO4 (n = 6 per group). Human labour and preterm chorioamnionitis significantly decreased cytoplasmic FoxO4 expression in placenta and/or choriodecidua. Knockdown of FoxO4 mRNA and protein in JEG-3 cells using siRNA was associated with decreased COX-2 mRNA expression concomitant with lower PGF secretion. However, in BeWo cells, siRNA inhibition of FoxO4 was not associated with inflammation, oxidative stress or apoptosis. In summary, human term labour and chorioamnionitis is characterised by lower FoxO4 mRNA and/or protein expression in placenta and/or choriodecidua. Although the exact role of FoxO4 in human pregnancy remains to be fully elucidated, our data demonstrate that it can regulate COX-2 expression and subsequent prostaglandin expression.
Keyword FoxO4
Fetal membranes
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 23 November 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2012 Collection
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 22 Feb 2012, 11:21:31 EST by System User on behalf of UQ Centre for Clinical Research