Study of beta endorphin metabolism in inflamed tissue, serum and trypsin solution by liquid chromatography-tandem mass spectrometric analysis

Dilanthi, H. M. D. R., Cabot, P. J., Shaw, P. N. and Hewavitharana, A. K. (2012) Study of beta endorphin metabolism in inflamed tissue, serum and trypsin solution by liquid chromatography-tandem mass spectrometric analysis. Analytical and Bioanalytical Chemistry, 402 6: 2089-2100. doi:10.1007/s00216-011-5686-8


Author Dilanthi, H. M. D. R.
Cabot, P. J.
Shaw, P. N.
Hewavitharana, A. K.
Title Study of beta endorphin metabolism in inflamed tissue, serum and trypsin solution by liquid chromatography-tandem mass spectrometric analysis
Journal name Analytical and Bioanalytical Chemistry   Check publisher's open access policy
ISSN 1618-2642
1618-2650
Publication date 2012-02-01
Sub-type Article (original research)
DOI 10.1007/s00216-011-5686-8
Volume 402
Issue 6
Start page 2089
End page 2100
Total pages 12
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
Beta endorphin (β-END) is recognised as one of the most significant endogenous neuropeptides, responsible for a wide range of biological activities in the body. However, within the body β-END is exposed to hydrolysis by a variety of enzymes. In this study, we investigated the metabolism and fragmentation pattern of β-END in rat inflamed tissue, in rat serum and in trypsin solution. β-END (1-31)-rat was incubated at 37 °C in each matrix for different incubation times. The resultant fragments were separated using a C4 column and detected by mass spectrometry using total ion current mode. Structural information for the fragments was elucidated using tandem mass spectrometry. Incubation of β-END (1-31)-rat in trypsin solution and in rat serum resulted in 8 and 13 fragments, respectively. Incubation in inflamed rat paw tissue resulted in 22 fragments at pH 7.4 and 26 fragments at pH 5.5. Some of these fragments were common to both pH values. The degradation of β-END (1-31)-rat in inflamed tissue at pH 5.5 was faster than that at pH 7.4. Secondary fragmentation of some larger primary fragments was also observed in this study.
Keyword Beta endorphin
Metabolism
Fragments
Inflamed tissue
LC-MS-MS
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Pharmacy Publications
 
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Created: Wed, 15 Feb 2012, 01:32:59 EST by Charna Kovacevic on behalf of School of Pharmacy