Lipid peptide core nanoparticles as multivalent vaccine candidates against Streptococcus pyogenes

Skwarczynski, Mariusz, Parhiz, Bibi Hamideh, Soltani, Fatemeh, Srinivasan, Saranya, Kamaruzaman, Khairul A., Lin, I-Chun and Toth, Istvan (2012) Lipid peptide core nanoparticles as multivalent vaccine candidates against Streptococcus pyogenes. Australian Journal of Chemistry, 65 1: 35-39. doi:10.1071/CH11292


Author Skwarczynski, Mariusz
Parhiz, Bibi Hamideh
Soltani, Fatemeh
Srinivasan, Saranya
Kamaruzaman, Khairul A.
Lin, I-Chun
Toth, Istvan
Title Lipid peptide core nanoparticles as multivalent vaccine candidates against Streptococcus pyogenes
Formatted title
Lipid peptide core nanoparticles as multivalent vaccine candidates against Streptococcus pyogenes
Journal name Australian Journal of Chemistry   Check publisher's open access policy
ISSN 0004-9425
1445-0038
Publication date 2012-01-01
Year available 2011
Sub-type Article (original research)
DOI 10.1071/CH11292
Volume 65
Issue 1
Start page 35
End page 39
Total pages 5
Place of publication Collingwood, VIC, Australia
Publisher CSIRO Publishing
Language eng
Formatted abstract
Traditional vaccine approaches for Group A streptococcus (GAS) infection are inadequate owing to the host’s production of cross-reactive antibodies that recognize not only the bacteria but also human tissue. To overcome this problem a peptide subunit-based vaccine was proposed, which would incorporate only minimal non-cross reactive epitopes. However, special delivery systems/adjuvants were required because short peptides are not immunogenic. In this study we have incorporated two epitopes from two different GAS proteins into a lipid core peptide (LCP) self-adjuvanting delivery system to achieve better protection against a wide range of GAS serotypes. Multivalent and monovalent constructs were synthesized with the help of an azide alkyne cycloaddition (click) reaction and their ability to self-assemble under aqueous conditions was examined. The compounds significantly differed in their ability to form small size nanoparticles, which are believed to be most appropriate for peptide-based subunit vaccine delivery. The LCP conjugates possessing two different epitopes, in contrast to monoepitopic constructs, formed small nanoparticles (5–15 nm) presumably owing to a suitable hydrophilic-hydrophobic balance of the molecules.
Keyword Group-A Streptococcus
M-protein
Intranasal vaccination
Delivery-system
Gas vaccine
T-cell
Design
Identification
Infections
Immunity
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 496600
Institutional Status UQ
Additional Notes Article first published online 3 October 2011.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Tue, 14 Feb 2012, 21:50:44 EST by Lucy O'Brien on behalf of School of Chemistry & Molecular Biosciences