SERRATE is required for intron suppression of RNA silencing in Arabidopsis

Christie, Michael and Carroll, Bernard J. (2011) SERRATE is required for intron suppression of RNA silencing in Arabidopsis. Plant Signaling and Behavior, 6 12: 2035-2037. doi:10.4161/psb.6.12.18238

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Author Christie, Michael
Carroll, Bernard J.
Title SERRATE is required for intron suppression of RNA silencing in Arabidopsis
Formatted title
SERRATE is required for intron suppression of RNA silencing in Arabidopsis
Journal name Plant Signaling and Behavior   Check publisher's open access policy
ISSN 1559-2316
1559-2324
Publication date 2011-12-01
Sub-type Article (original research)
DOI 10.4161/psb.6.12.18238
Volume 6
Issue 12
Start page 2035
End page 2037
Total pages 3
Place of publication Austin, TX, United States
Publisher Landes Biosciences
Language eng
Formatted abstract
Transposons and viruses are generally devoid of introns and are prime targets for small interfering RNAs (siRNAs) and RNA silencing. Conversely, endogenous genes often contain introns and are not usually subjected to post-transcriptional gene silencing by siRNAs. In a recent study, we reported that efficient intron splicing directly suppresses siRNA biogenesis and RNA silencing of a Green Fluorescence Protein (GFP) transgene. Splicing-mediated suppression of GFP silencing was dependent on ABH1, the Arabidopsis ortholog of human mRNA cap-binding protein 80. Furthermore, genome-wide analyses of Arabidopsis small RNA libraries showed that exons from intron-containing genes accumulate less small RNAs than exons from intronless genes. Our in silico analysis therefore suggested that intron splicing has a fundamental role in protecting endogenous genes from becoming templates for siRNA biogenesis and RNA silencing. Here, we show that SERRATE (SE) is also required for splicing-mediated suppression of RNA silencing in Arabidopsis. SE encodes a zinc finger protein that, like ABH1, functions in micro-RNA (miRNA) biogenesis and intron splicing. The implications of our findings are also discussed in a broader context.
Q-Index Code CX
Q-Index Status Confirmed Code
Institutional Status UQ
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Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
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School of Chemistry and Molecular Biosciences
 
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Created: Tue, 07 Feb 2012, 21:55:10 EST by Lucy O'Brien on behalf of School of Chemistry & Molecular Biosciences