Tuba and N-WASP function cooperatively to position the central lumen during epithelial cyst morphogenesis

Kovacs, Eva M., Verma, Suzie, Thomas, Steven G. and Yap, Alpha S. (2011) Tuba and N-WASP function cooperatively to position the central lumen during epithelial cyst morphogenesis. Cell Adhesion and Migration, 5 4: 344-350. doi:10.4161/cam.5.4.16717

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Author Kovacs, Eva M.
Verma, Suzie
Thomas, Steven G.
Yap, Alpha S.
Title Tuba and N-WASP function cooperatively to position the central lumen during epithelial cyst morphogenesis
Journal name Cell Adhesion and Migration   Check publisher's open access policy
ISSN 1933-6918
1933-6926
Publication date 2011-07-01
Sub-type Article (original research)
DOI 10.4161/cam.5.4.16717
Volume 5
Issue 4
Start page 344
End page 350
Total pages 7
Place of publication Austin, TX, U.S.A.
Publisher Landes Bioscience
Collection year 2012
Language eng
Formatted abstract
The process of epithelial lumenogenesis requires coordination of a network of signaling machinery communicated to each cell through subsequent cell divisions. Formation of a single hollow lumen has previously been shown to require Tuba, a Cdc42 GEF, for Cdc42 activation and correct spindle orientation. Using a Caco-2 model of lumenogenesis, we show that knockdown (KD) of the actin regulator N-WASP, causes a multilumen phenotype similar to Tuba KD. Defects in lumenogenesis in Tuba KD and N-WASP KD cells are observed at the two cell stage with inappropriate marking of the pre-apical patch (PAP) – the precursor to lumen formation. Strikingly, both Tuba and N-WASP depend on each other for localization to the PAP. We conclude that N-WASP functions cooperatively with Tuba to facilitate lumenogenesis and this requires the polyproline region of N-WASP.
Keyword Lumen
M-WASP
Tuba
E-cadherin
Pre-apical patch
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Publication date: July/August 2011. Special Issue: "Special Focus on Recent advances in cell-cell adhesion".

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
 
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Created: Tue, 31 Jan 2012, 22:03:10 EST by Susan Allen on behalf of Institute for Molecular Bioscience