Multiplex PCR for detection of acquired carbapenemase genes

Poirel, Laurent, Walsh, Timothy R., Cuvillier, Vincent and Nordmann, Patrice (2011) Multiplex PCR for detection of acquired carbapenemase genes. Diagnostic Microbiology and Infectious Disease, 70 1: 119-123. doi:10.1016/j.diagmicrobio.2010.12.002

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Author Poirel, Laurent
Walsh, Timothy R.
Cuvillier, Vincent
Nordmann, Patrice
Title Multiplex PCR for detection of acquired carbapenemase genes
Journal name Diagnostic Microbiology and Infectious Disease   Check publisher's open access policy
ISSN 0732-8893
1879-0070
Publication date 2011-05-01
Sub-type Article (original research)
DOI 10.1016/j.diagmicrobio.2010.12.002
Open Access Status Not yet assessed
Volume 70
Issue 1
Start page 119
End page 123
Total pages 5
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Formatted abstract
A rapid and reliable PCR-based technique was developed for detection of genes encoding carbapenemases belonging to different classes. Primers were designed to amplify the following 11 genes: blaIMP, blaVIM, blaNDM, blaSPM, blaAIM, blaDIM, blaGIM, blaSIM blaKPC, blaBIC, and blaOXA-48 . Three different multiplex reaction mixtures were defined and evaluated for the detection of all these 11 genes. Using optimized conditions, each reaction mixture allowed to identify the respective genes, with PCR giving distinct amplicon sizes corresponding to the different genes for each mixture. We reported here a rapid and reliable technique for screening all clinically relevant carbapenemase genes. 
Keyword Carbapenem
Metallo-beta-lactamase
Multiplex
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Non HERDC
 
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Citation counts: TR Web of Science Citation Count  Cited 425 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 458 times in Scopus Article | Citations
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Created: Tue, 31 Jan 2012, 21:17:25 EST by Roheen Gill on behalf of UQ Centre for Clinical Research