Sex specific changes in placental growth and MAPK following short term maternal dexamethasone exposure in the mouse

Cuffe, J. S. M., Dickinson, H., Simmons, D. G. and Moritz, K. M. (2011) Sex specific changes in placental growth and MAPK following short term maternal dexamethasone exposure in the mouse. Placenta, 32 12: 981-989. doi:10.1016/j.placenta.2011.09.009


Author Cuffe, J. S. M.
Dickinson, H.
Simmons, D. G.
Moritz, K. M.
Title Sex specific changes in placental growth and MAPK following short term maternal dexamethasone exposure in the mouse
Journal name Placenta   Check publisher's open access policy
ISSN 0143-4004
1532-3102
Publication date 2011-12-01
Sub-type Article (original research)
DOI 10.1016/j.placenta.2011.09.009
Volume 32
Issue 12
Start page 981
End page 989
Total pages 9
Place of publication Oxford, England, U. K.
Publisher Elsevier
Language eng
Formatted abstract
Objectives
Maternal glucocorticoid (GC) exposure during pregnancy can alter fetal development and program the onset of disease in adult offspring. The placenta helps protect the fetus from excess GC exposure but is itself susceptible to maternal insults and may be involved in sex dependant regulation of fetal programming. This study aimed to investigate the effects of maternal GC exposure on the developing placenta.
Study design and main outcome measures
Pregnant mice were treated with dexamethasone (DEX-1 μg/kg/h) or saline (SAL) for 60 h via minipump beginning at E12.5. Placentas were collected at E14.5 and E17.5 and the expression of growth factors and placental transporters examined by real-time PCR and/or Western blot. Histological analysis was performed to assess for morphological changes.
Results
At E14.5, DEX exposed male and female fetuses had a lower weight compared to SAL animals but placental weight was lower in females only. Hsd11b2 and Vegfa gene expression was increased and MAPK1 protein expression decreased in the placentas of females only. At E17.5 placental and fetal body weights were similar and differences in MAPK were no longer present although HSD11B2 protein was elevated in placentas of DEX females. Levels of glucose or amino acid transporters were unaffected.
Conclusions
Results suggest sex specific responses to maternal GCs within the placenta. Decreased levels of MAPK protein in placentas of female fetuses suggest alterations in the MAPK pathway may contribute to the lower placental weights in this sex. This may contribute towards sex specific fetal programming of adult disease.
Keyword Fetal programming
Vegf
Hsd11b2
Glucocorticoids
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 5 October 2011.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Biomedical Sciences Publications
 
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