Striking denervation of neuromuscular junctions without lumbar motoneuron loss in geriatric mouse muscle

Chai, Ruth Jinfen, Vukovic, Jana, Dunlop, Sarah, Grounds, Miranda D. and Shavlakadze, Thea (2011) Striking denervation of neuromuscular junctions without lumbar motoneuron loss in geriatric mouse muscle. PLoS One, 6 12: e28090.1-e28090.11. doi:10.1371/journal.pone.0028090


Author Chai, Ruth Jinfen
Vukovic, Jana
Dunlop, Sarah
Grounds, Miranda D.
Shavlakadze, Thea
Title Striking denervation of neuromuscular junctions without lumbar motoneuron loss in geriatric mouse muscle
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2011-12-01
Year available 2011
Sub-type Article (original research)
DOI 10.1371/journal.pone.0028090
Open Access Status DOI
Volume 6
Issue 12
Start page e28090.1
End page e28090.11
Total pages 11
Place of publication San Francisco, CA, United Sates
Publisher Public Library of Science
Language eng
Subject 1100 Agricultural and Biological Sciences
1300 Biochemistry, Genetics and Molecular Biology
2700 Medicine
Abstract Reasons for the progressive age-related loss of skeletal muscle mass and function, namely sarcopenia, are complex. Few studies describe sarcopenia in mice, although this species is the mammalian model of choice for genetic intervention and development of pharmaceutical interventions for muscle degeneration. One factor, important to sarcopenia-associated neuromuscular change, is myofibre denervation. Here we describe the morphology of the neuromuscular compartment in young (3 month) compared to geriatric (29 month) old female C57Bl/6J mice. There was no significant difference in the size or number of motoneuron cell bodies at the lumbar level (L1-L5) of the spinal cord at 3 and 29 months. However, in geriatric mice, there was a striking increase (by ~2.5 fold) in the percentage of fully denervated neuromuscular junctions (NMJs) and associated deterioration of Schwann cells in fast extensor digitorum longus (EDL), but not in slow soleus muscles. There were also distinct changes in myofibre composition of lower limb muscles (tibialis anterior (TA) and soleus) with a shift at 29 months to a faster phenotype in fast TA muscle and to a slower phenotype in slow soleus muscle. Overall, we demonstrate complex changes at the NMJ and muscle levels in geriatric mice that occur despite the maintenance of motoneuron cell bodies in the spinal cord. The challenge is to identify which components of the neuromuscular system are primarily responsible for the marked changes within the NMJ and muscle, in order to selectively target future interventions to reduce sarcopenia.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
MULTIDISCIPLINARY SCIENCES
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 572654
Institutional Status UQ
Additional Notes Article no. e28090, pp.1-11

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2012 Collection
 
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Created: Thu, 19 Jan 2012, 02:38:37 EST by Jana Vukovic on behalf of Queensland Brain Institute