Cbx2, a polycomb group gene, is required for Sry gene expression in mice

Katoh-Fukui, Yuko, Miyabayashi, Kanako, Komatsu, Tomoko, Owaki. Akiko, Baba, Takashi, Shima, Yuichi, Kidokoro, Tomohide, Kanai, Yoshiakira, Schedl, Andreas, Wilhelm, Dagmar, Koopman, Peter, Okuno, Yasushi and Morohashi, Ken-ichirou (2012) Cbx2, a polycomb group gene, is required for Sry gene expression in mice. Endocrinology, 153 2: 913-924. doi:10.1210/en.2011-1055

Author Katoh-Fukui, Yuko
Miyabayashi, Kanako
Komatsu, Tomoko
Owaki. Akiko
Baba, Takashi
Shima, Yuichi
Kidokoro, Tomohide
Kanai, Yoshiakira
Schedl, Andreas
Wilhelm, Dagmar
Koopman, Peter
Okuno, Yasushi
Morohashi, Ken-ichirou
Title Cbx2, a polycomb group gene, is required for Sry gene expression in mice
Formatted title
Cbx2, a polycomb group gene, is required for Sry gene expression in mice
Journal name Endocrinology   Check publisher's open access policy
ISSN 0013-7227
Publication date 2012-02-01
Year available 2011
Sub-type Article (original research)
DOI 10.1210/en.2011-1055
Open Access Status Not Open Access
Volume 153
Issue 2
Start page 913
End page 924
Total pages 12
Place of publication Chevy Chase, MD, United States
Publisher The Endocrine Society
Language eng
Formatted abstract
Mice lacking the function of the polycomb group protein CBX2 (chromobox homolog 2; also known as M33) show defects in gonadal, adrenal, and splenic development. In particular, XY knockout (KO) mice develop ovaries but not testes, and the gonads are hypoplastic in both sexes. However, how CBX2 regulates development of these tissues remains largely unknown. In the present study, we used microarray, RT-PCR, and immunohistochemical analyses to show that the expression of Sry, Sox9, Lhx9, Ad4BP/SF-1, Dax-1, Gata4, Arx, and Dmrt1, genes encoding transcription factors essential for gonadal development, is affected in Cbx2 KO gonads. Male-to-female sex reversal in Cbx2 KO mice was rescued by crossing them with transgenic mice displaying forced expression of Sry or Sox9. However, testes remained hypoplastic in these mice, indicating that the size and the sex of the gonad are determined by different sets of genes. Our study implicates Cbx2 in testis differentiation through regulating Sry gene expression.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online before print December 20, 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
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Created: Wed, 18 Jan 2012, 00:10:44 EST by Susan Allen on behalf of Institute for Molecular Bioscience