Elevated levels of Dickkopf-related protein 3 in seminal plasma of prostate cancer patients

Zenzmaier, Christoph, Heitz, Martin, Klocker, Helmut, Buck, Marion, Gardiner, Robert A. and Berger, Peter (2011) Elevated levels of Dickkopf-related protein 3 in seminal plasma of prostate cancer patients. Journal of Translational Medicine, 9 1: 193.1-193.7. doi:10.1186/1479-5876-9-193

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Author Zenzmaier, Christoph
Heitz, Martin
Klocker, Helmut
Buck, Marion
Gardiner, Robert A.
Berger, Peter
Title Elevated levels of Dickkopf-related protein 3 in seminal plasma of prostate cancer patients
Journal name Journal of Translational Medicine   Check publisher's open access policy
ISSN 1479-5876
Publication date 2011-11-01
Sub-type Article (original research)
DOI 10.1186/1479-5876-9-193
Open Access Status DOI
Volume 9
Issue 1
Start page 193.1
End page 193.7
Total pages 7
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background: Expression of Dkk-3, a secreted putative tumor suppressor, is altered in age-related proliferative disorders of the human prostate. We now investigated the suitability of Dkk-3 as a diagnostic biomarker for prostate cancer (PCa) in seminal plasma (SP).
Methods: SP samples were obtained from 81 patients prior to TRUS-guided prostate biopsies on the basis of elevated serum prostate-specific antigen (PSA; > 4 ng/mL) levels and/or abnormal digital rectal examination. A sensitive indirect immunoenzymometric assay for Dkk-3 was developed and characterized in detail. SP Dkk-3 and PSA levels were determined and normalized to total SP protein. The diagnostic accuracies of single markers including serum PSA and multivariate models to discriminate patients with positive (N = 40) and negative (N = 41) biopsy findings were investigated.
Results: Biopsy-confirmed PCa showed significantly higher SP Dkk-3 levels (100.9 ± 12.3 vs. 69.2 ± 9.4 fmol/mg; p = 0.026). Diagnostic accuracy (AUC) of SP Dkk-3 levels (0.633) was enhanced in multivariate models by including serum PSA (model A; AUC 0.658) or both, serum and SP PSA levels (model B; AUC 0.710). In a subpopulation with clinical follow-up > 3 years post-biopsy to ensure veracity of negative biopsy status (positive biopsy N = 21; negative biopsy N = 25) AUCs for SP Dkk-3, model A and B increased to 0.667, 0.724 and 0.777, respectively.
Conclusions: In multivariate models to detect PCa, inclusion of SP Dkk-3 levels, which were significantly elevated in biopsy-confirmed PCa patients, improved the diagnostic performance compared with serum PSA only.
Keyword Dickkopf-3
Prostate-specific antigen
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article # 193

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2012 Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
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