Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro

Xiang, Dong-xi, Chen, Qian, Pang, Lin and Zheng, Cong-long (2011) Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro. Journal of Virological Methods, 178 1-2: 137-142. doi:10.1016/j.jviromet.2011.09.003


Author Xiang, Dong-xi
Chen, Qian
Pang, Lin
Zheng, Cong-long
Title Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro
Formatted title
Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro
Journal name Journal of Virological Methods   Check publisher's open access policy
ISSN 0166-0934
1879-0984
Publication date 2011-12-01
Sub-type Article (original research)
DOI 10.1016/j.jviromet.2011.09.003
Open Access Status Not yet assessed
Volume 178
Issue 1-2
Start page 137
End page 142
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Subject 2406 Virology
Abstract Silver nanoparticles have demonstrated efficient inhibitory activities against human immunodeficiency virus (HIV) and hepatitis B virus (HBV). However, the effects of silver nanoparticles against H1N1 influenza A virus remain unexplored. In this study, the interaction of silver nanoparticles with H1N1 influenza A virus was investigated. Silver nanoparticles with mean particle diameters of 10 nm were prepared for the hemagglutination inhibition test, the embryo inoculation assay, and the Mosmann-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, where these tests were used to determine the inhibitory activity of silver nanoparticles on H1N1 influenza A virus. MDCK cells were used as the infection model. Electron microscopy analysis and flow cytometry assay were used to determine whether silver nanoparticles could reduce H1N1 influenza A virus-induced apoptosis in MDCK cells. This study demonstrates that silver nanoparticles have anti-H1N1 influenza A virus activities. The inhibitory effects of silver nanoparticles on influenza A virus may be a novel clinical strategy for the prevention of influenza virus infection during the early dissemination stage of the virus.
Keyword Silver nanoparticles
H1N1 influenza A virus
Antiviral activity
Escherichia-coli
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
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