Adenoviral vector-mediated rescue of the OMP-null phenotype in vivo

Ivic, L, Pyrski, MM, Margolis, JW, Richards, LJ, Firestein, S and Margolis, FL (2000) Adenoviral vector-mediated rescue of the OMP-null phenotype in vivo. Nature Neuroscience, 3 11: 1113-1120.

Author Ivic, L
Pyrski, MM
Margolis, JW
Richards, LJ
Firestein, S
Margolis, FL
Title Adenoviral vector-mediated rescue of the OMP-null phenotype in vivo
Journal name Nature Neuroscience   Check publisher's open access policy
ISSN 1097-6256
Publication date 2000-11-01
Year available 2000
Sub-type Article (original research)
Open Access Status Not yet assessed
Volume 3
Issue 11
Start page 1113
End page 1120
Total pages 8
Place of publication NEW YORK
Language eng
Abstract The use of gene deletion by homologous recombination to determine gene or protein function has wide application in vertebrate neurobiology. An ideal complement to gene deletion would be subsequent gene replacement to demonstrate re-acquisition of function. Here we used an adenoviral vector to replace the olfactory marker protein (OMP) gene in olfactory receptor neurons of adult OMP-null mice and demonstrated the subsequent re-acquisition of function. Our results show that short-term expression of OMP restores the kinetics of electrophysiological responses of OMP-null mice to those of the control phenotype. This adenoviral-mediated rescue of the OMP-null phenotype is consistent with involvement of OMP in olfactory transduction.
Keyword Olfactory Marker Protein
Gene Deletion Causes
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID DCR01-03112
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
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Citation counts: TR Web of Science Citation Count  Cited 47 times in Thomson Reuters Web of Science Article | Citations
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