Lineage Specification of Neuronal Precursors in the Mouse Spinal-Cord

Richards, LJ, Murphy, M, Dutton, R, Kilpatrick, TJ, Puche, AC, Tan, SS, Talman, PS and Bartlett, PF (1995) Lineage Specification of Neuronal Precursors in the Mouse Spinal-Cord. Proceedings of the National Academy of Sciences of the United States of America, 92 22: 10079-10083. doi:10.1073/pnas.92.22.10079


Author Richards, LJ
Murphy, M
Dutton, R
Kilpatrick, TJ
Puche, AC
Tan, SS
Talman, PS
Bartlett, PF
Title Lineage Specification of Neuronal Precursors in the Mouse Spinal-Cord
Journal name Proceedings of the National Academy of Sciences of the United States of America   Check publisher's open access policy
ISSN 0027-8424
Publication date 1995-10-01
Year available 1995
Sub-type Article (original research)
DOI 10.1073/pnas.92.22.10079
Open Access Status Not Open Access
Volume 92
Issue 22
Start page 10079
End page 10083
Total pages 5
Place of publication WASHINGTON
Publisher NATL ACAD SCIENCES
Language eng
Abstract We have investigated the differentiation potential of precursor cells within the developing spinal cord of mice and have shown that spinal cord cells from embryonic day 10 specifically give rise to neurons when plated onto an astrocytic monolayer, Ast-1. These neurons had the morphology of motor neurons and >83% expressed the motor neuron markers choline acetyltransferase, peripherin, calcitonin gene-related peptide, and L-14. By comparison, <10% of the neurons arising on monolayers of other neural cell lines or 3T3 fibroblasts had motor neuron characteristics. Cells derived from dorsal, intermediate, and ventral regions of the spinal cord all behaved similarly and gave rise to motor neuron-like cells when plated onto Ast-1. By using cells that expressed the lacZ reporter gene, it was shown that >93% of cells present on the Ast-1 monolayers were motor neuron-like. Time-lapse analysis revealed that the precursors on the Ast-1 monolayers gave rise to neurons either directly or following a single cell division. Together, these results indicate that precursors in the murine spinal cord can be induced to differentiate into the motor neuron phenotype by factors produced by Ast-1 cells, suggesting that a similar factor(s) produced by cells akin to Ast-1 may regulate motor neuron differentiation in vivo.
Keyword Neural Progenitors
Cell Plasticity
Neuronal Differentiation
Astrocytes
Lineage Determination
Central-Nervous-System
Choline-Acetyltransferase
Neural-Tube
Chick-Embryo
Floor Plate
Monoclonal-Antibodies
Cell Pattern
Adult-Rat
C-Myc
Differentiation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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