Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP

Moriyoshi, K, Richards, LJ, Akazawa, C, OLeary, DDM and Nakanishi, S (1996) Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP. Neuron, 16 2: 255-260. doi:10.1016/S0896-6273(00)80044-6


Author Moriyoshi, K
Richards, LJ
Akazawa, C
OLeary, DDM
Nakanishi, S
Title Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP
Journal name Neuron   Check publisher's open access policy
ISSN 0896-6273
Publication date 1996-02-01
Year available 1996
Sub-type Article (original research)
DOI 10.1016/S0896-6273(00)80044-6
Open Access Status DOI
Volume 16
Issue 2
Start page 255
End page 260
Total pages 6
Place of publication CAMBRIDGE
Publisher CELL PRESS
Language eng
Abstract We describe an experimental system to visualize the soma and processes of mammalian neurons and glia in living and fixed preparations by using a recombinant adenovirus vector to transfer the jellyfish green fluorescent protein (GFP) into postmitotic neural cells both in vitro and in vivo. We have introduced several modifications of GFP that enhance its fluorescence intensity in mammalian axons and dendrites. This method should be useful for studying the dynamic processes of cell migration and the development of neuronal connections, as well as for analyzing the function of exogenous genes introduced into cells using the adenovirus vector.
Keyword Neurons
Vector
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID NS 31558
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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