Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite

Warnecke, Falk, Luginbuehl, Peter, Ivanova, Natalia, Ghassemian, Majid, Richardson, Toby H., Stege, Justin T., Cayouette, Michelle, McHardy, Alice C., Djordjevic, Gordana, Aboushadi, Nahla, Sorek, Rotem, Tringe, Susannah G., Podar, Mircea, Martin, Hector Garcia, Kunin, Victor, Dalevi, Daniel, Madejska, Julita, Kirton, Edward, Platt, Darren, Szeto, Ernest, Salamov, Asaf, Barry, Kerrie, Mikhailova, Natalia, Kyrpides, Nikos C., Matson, Eric G., Ottesen, Elizabeth A., Zhang, Xinning, Hernandez, Myriam, Murillo, Catalina, Acosta, Luis G., Rigoutsos, Isidore, Tamayo, Giselle, Green, Brian D., Chang, Cathy, Rubin, Edward M., Mathur, Eric J., Robertson, Dan E., Hugenholtz, Philip and Leadbetter, Jared R. (2007) Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite. Nature, 450 7169: 560-565. doi:10.1038/nature06269


Author Warnecke, Falk
Luginbuehl, Peter
Ivanova, Natalia
Ghassemian, Majid
Richardson, Toby H.
Stege, Justin T.
Cayouette, Michelle
McHardy, Alice C.
Djordjevic, Gordana
Aboushadi, Nahla
Sorek, Rotem
Tringe, Susannah G.
Podar, Mircea
Martin, Hector Garcia
Kunin, Victor
Dalevi, Daniel
Madejska, Julita
Kirton, Edward
Platt, Darren
Szeto, Ernest
Salamov, Asaf
Barry, Kerrie
Mikhailova, Natalia
Kyrpides, Nikos C.
Matson, Eric G.
Ottesen, Elizabeth A.
Zhang, Xinning
Hernandez, Myriam
Murillo, Catalina
Acosta, Luis G.
Rigoutsos, Isidore
Tamayo, Giselle
Green, Brian D.
Chang, Cathy
Rubin, Edward M.
Mathur, Eric J.
Robertson, Dan E.
Hugenholtz, Philip
Leadbetter, Jared R.
Title Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite
Journal name Nature   Check publisher's open access policy
ISSN 0028-0836
1476-4687
Publication date 2007-11-22
Year available 2007
Sub-type Article (original research)
DOI 10.1038/nature06269
Open Access Status Not yet assessed
Volume 450
Issue 7169
Start page 560
End page 565
Total pages 6
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract From the standpoints of both basic research and biotechnology, there is considerable interest in reaching a clearer understanding of the diversity of biological mechanisms employed during lignocellulose degradation. Globally, termites are an extremely successful group of wood-degrading organisms(1) and are therefore important both for their roles in carbon turnover in the environment and as potential sources of biochemical catalysts for efforts aimed at converting wood into biofuels. Only recently have data supported any direct role for the symbiotic bacteria in the gut of the termite in cellulose and xylan hydrolysis(2). Here we use a metagenomic analysis of the bacterial community resident in the hindgut paunch of a wood-feeding 'higher' Nasutitermes species ( which do not contain cellulose-fermenting protozoa) to show the presence of a large, diverse set of bacterial genes for cellulose and xylan hydrolysis. Many of these genes were expressed in vivo or had cellulase activity in vitro, and further analyses implicate spirochete and fibrobacter species in gut lignocellulose degradation. New insights into other important symbiotic functions including H-2 metabolism, CO2-reductive acetogenesis and N-2 fixation are also provided by this first system-wide gene analysis of a microbial community specialized towards plant lignocellulose degradation. Our results underscore how complex even a 1-mu l environment can be.
Formatted abstract
From the standpoints of both basic research and biotechnology, there is considerable interest in reaching a clearer understanding of the diversity of biological mechanisms employed during lignocellulose degradation. Globally, termites are an extremely successful group of wood-degrading organisms1 and are therefore important both for their roles in carbon turnover in the environment and as potential sources of biochemical catalysts for efforts aimed at converting wood into biofuels. Only recently have data supported any direct role for the symbiotic bacteria in the gut of the termite in cellulose and xylan hydrolysis2. Here we use a metagenomic analysis of the bacterial community resident in the hindgut paunch of a wood-feeding ‘higher’ Nasutitermes species (which do not contain cellulose-fermenting protozoa) to show the presence of a large, diverse set of bacterial genes for cellulose and xylan hydrolysis. Many of these genes were expressed in vivo or had cellulase activity in vitro, and further analyses implicate spirochete and fibrobacter species in gut lignocellulose degradation. New insights into other important symbiotic functions including H2 metabolism, CO2-reductive acetogenesis and N2 fixation are also provided by this first system-wide gene analysis of a microbial community specialized towards plant lignocellulose degradation. Our results underscore how complex even a 1-μl environment can be.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
MULTIDISCIPLINARY SCIENCES
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published under Letters

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 705 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 715 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 28 Nov 2011, 19:22:28 EST by Hong Lee on behalf of School of Chemistry & Molecular Biosciences