A novel role for hSMG-1 in stress granule formation

Brown, James, L., Roberts, Tara L., Richards, Renee, Woods, Rick, Birrell, Geoff, Lim, Y. C., Ohno, Shigeo, Yamashita, Akio, Abraham, Robert T., Gueven, Nuri and Lavin, Martin F. (2011) A novel role for hSMG-1 in stress granule formation. Molecular and Cellular Biology, 31 22: 4417-4429. doi:10.1128/MCB.05987-11

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Author Brown, James, L.
Roberts, Tara L.
Richards, Renee
Woods, Rick
Birrell, Geoff
Lim, Y. C.
Ohno, Shigeo
Yamashita, Akio
Abraham, Robert T.
Gueven, Nuri
Lavin, Martin F.
Title A novel role for hSMG-1 in stress granule formation
Journal name Molecular and Cellular Biology   Check publisher's open access policy
ISSN 0270-7306
1098-5549
Publication date 2011-11-01
Year available 2011
Sub-type Article (original research)
DOI 10.1128/MCB.05987-11
Open Access Status File (Publisher version)
Volume 31
Issue 22
Start page 4417
End page 4429
Total pages 13
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract hSMG-1 is a member of the phosphoinositide 3 kinase-like kinase (PIKK) family with established roles in nonsense-mediated decay (NMD) of mRNA containing premature termination codons and in genotoxic stress responses to DNA damage. We report here a novel role for hSMG-1 in cytoplasmic stress granule (SG) formation. Exposure of cells to stress causing agents led to the localization of hSMG-1 to SG, identified by colocalization with TIA-1, G3BP1, and eIF4G. hSMG-1 small interfering RNA and the PIKK inhibitor wortmannin prevented formation of a subset of SG, while specific inhibitors of ATM, DNA-PKcs , or mTOR had no effect. exposure of cells to H2O2 and sodium arsenite induced (S/T)Q phosphorylation of proteins. While Upf2 and Upf1, an essential substrate for hSMG-1 in NMD, were present in SG, NMD-specific Upf1 phosphorylation was not detected in SG, indicating hSMG-1’s role in SG is separate from classical NMD. Thus, SG formation appears more complex than originally envisaged and hSMG-1 plays a central role in this process.
Keyword Messenger RNA Decay
Protein Kinase
SMG-1 Kinase
Surveillance Complex
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2012 Collection
 
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