A role for BK channels in heart rate regulation in rodents

Imlach, Wendy L., Finch, Sarah C., Miller, John H., Meredith, Andrea L. and Dalziel, Julie E. (2010) A role for BK channels in heart rate regulation in rodents. PLoS One, 5 1: . doi:10.1371/journal.pone.0008698

Author Imlach, Wendy L.
Finch, Sarah C.
Miller, John H.
Meredith, Andrea L.
Dalziel, Julie E.
Title A role for BK channels in heart rate regulation in rodents
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2010-01-14
Sub-type Article (original research)
DOI 10.1371/journal.pone.0008698
Open Access Status DOI
Volume 5
Issue 1
Total pages 7
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Formatted abstract
The heart generates and propagates action potentials through synchronized activation of ion channels allowing inward Na+ and Ca2+ and outward K+ currents. There are a number of K+ channel types expressed in the heart that play key roles in regulating the cardiac cycle. Large conductance calcium-activated potassium (BK) ion channels are not thought to be directly involved in heart function. Here we present evidence that heart rate can be significantly reduced by inhibiting the activity of BK channels. Agents that specifically inhibit BK channel activity, including paxilline and lolitrem B, slowed heart rate in conscious wild-type mice by 30% and 42%, respectively. Heart rate of BK channel knock-out mice (Kcnma1−/−) was not affected by these BK channel inhibitors, suggesting that the changes to heart rate were specifically mediated through BK channels. The possibility that these effects were mediated through BK channels peripheral to the heart was ruled out with experiments using isolated, perfused rat hearts, which showed a significant reduction in heart rate when treated with the BK channel inhibitors paxilline (1 µM), lolitrem B (1 µM), and iberiotoxin (0.23 µM), of 34%, 60%, and 42%, respectively. Furthermore, paxilline was shown to decrease heart rate in a dose-dependent manner. These results implicate BK channels located in the heart to be directly involved in the regulation of heart rate.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article # e8698

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
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