Organization of ETRAMPs and EXP-1 at the parasite-host cell interface of malaria parasites

Spielmann, Tobias, Gardiner, Donald L., Beck, Hans-Peter, Trenholme, Katharine R and Kemp, David J. (2006) Organization of ETRAMPs and EXP-1 at the parasite-host cell interface of malaria parasites. Molecular Microbiology, 59 3: 779-794. doi:10.1111/j.1365-2958.2005.04983.x

Author Spielmann, Tobias
Gardiner, Donald L.
Beck, Hans-Peter
Trenholme, Katharine R
Kemp, David J.
Title Organization of ETRAMPs and EXP-1 at the parasite-host cell interface of malaria parasites
Journal name Molecular Microbiology   Check publisher's open access policy
ISSN 0950-382X
Publication date 2006-02-01
Year available 2005
Sub-type Article (original research)
DOI 10.1111/j.1365-2958.2005.04983.x
Open Access Status DOI
Volume 59
Issue 3
Start page 779
End page 794
Total pages 16
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
The parasite–host cell interface is a key compartment of vacuolated intracellular pathogens but little is known about its molecular composition and architecture. We used in vivo cross-linking to analyse the parasite–host cell interface of asexual stages of the most virulent human malaria parasite Plasmodium falciparum. We show that the integral membrane protein members of the early transcribed membrane protein (ETRAMP) family and exported protein 1 (EXP-1), which are components of the parasite–host cell interface, form complexes of oligomeric arrays in this compartment. The most notable feature is that each ETRAMP member and EXP-1 define separate arrays, demonstrating that the protein distribution in this membrane is non-random. Each of three recombinant ETRAMPs readily oligomerized in bacterial membranes, confirming that these arrays can form independently of other Plasmodium proteins. We propose that the malaria parasite–host cell interface contains patches of integral membrane proteins forming a mosaic of different microdomains in this membrane.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article first published online: 30 NOV 2005

Document type: Journal Article
Sub-type: Article (original research)
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