Isoflavonoid photoprotection in mouse and human skin is dependent on metallothionein

Widyarini, Sitarina, Allanson, Munif, Gallagher, Nerida L., Pedley, Julie, Boyle, Glen M., Parsons, Peter G., Whiteman, David C., Walker, Catherine and Reeve, Vivienne E. (2006) Isoflavonoid photoprotection in mouse and human skin is dependent on metallothionein. Journal of Investigative Dermatology, 126 1: 198-204. doi:10.1038/sj.jid.5700013

Author Widyarini, Sitarina
Allanson, Munif
Gallagher, Nerida L.
Pedley, Julie
Boyle, Glen M.
Parsons, Peter G.
Whiteman, David C.
Walker, Catherine
Reeve, Vivienne E.
Title Isoflavonoid photoprotection in mouse and human skin is dependent on metallothionein
Journal name Journal of Investigative Dermatology   Check publisher's open access policy
ISSN 0022-202X
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1038/sj.jid.5700013
Open Access Status Not yet assessed
Volume 126
Issue 1
Start page 198
End page 204
Total pages 7
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract Previous studies report that selected topical isoflavonoids are immunoprotective in both mice and humans, when applied following UV irradiation. Isoflavonoids have documented antioxidant activity, but their mechanism of immunomodulation remains unclear. This study examines whether photoimmunoprotection by the isoflavonoids might result from their interaction with one cutaneous antioxidant known to modulate UV photodamage, metallothionein (MT). In mice bearing a null mutation for MT-I and -II, we found that immunoprotection by the isoflavonoid 4′,7-dihydroxyisoflavane (equol) against solar-simulated UV radiation (SSUV) or exogenous cis-urocanic acid was abrogated. Topical equol did not activate MT expression in normal mouse skin, but markedly enhanced the increase in MT expression in murine epidermis following SSUV irradiation. Normal human skin, unlike murine, expressed MT in the basal epidermis. Following SSUV irradiation, topical application of the related synthetic isoflavonoid NV-07α to human skin also markedly enhanced epidermal MT expression. The NV-07α has been reported previously to protect humans against the UV suppression of Mantoux reactions. Thus, epidermal MT expression appears to protect against photoimmunosuppression in both human and mouse skin. We speculate that equol and its related derivative NV-07α may activate the MT gene synergistically with SSUV, to produce the enhanced immunoprotective effect.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
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