Jmjd6 catalyses lysyl-hydroxylation of U2AF65, a protein associated with RNA splicing

Webby, Celia J., Wolf, Alexander, Gromak, Natalia, Dreger, Mathias, Schmitz, Corinna, Butler, Danica S., Yates III, John R., Delahunty, Claire M., Hahn, Phillip, Lengeling, Andreas, Proudfoot, Nicholas J., Böttger, Angelika and Schofield, Christopher J. (2009) Jmjd6 catalyses lysyl-hydroxylation of U2AF65, a protein associated with RNA splicing. Science, 325 5936: 90-93. doi:10.1126/science.1175865


Author Webby, Celia J.
Wolf, Alexander
Gromak, Natalia
Dreger, Mathias
Schmitz, Corinna
Butler, Danica S.
Yates III, John R.
Delahunty, Claire M.
Hahn, Phillip
Lengeling, Andreas
Proudfoot, Nicholas J.
Böttger, Angelika
Schofield, Christopher J.
Title Jmjd6 catalyses lysyl-hydroxylation of U2AF65, a protein associated with RNA splicing
Journal name Science   Check publisher's open access policy
ISSN 0036-8075
1095-9203
Publication date 2009-07-03
Sub-type Article (original research)
DOI 10.1126/science.1175865
Volume 325
Issue 5936
Start page 90
End page 93
Total pages 4
Place of publication Washington, DC, United States
Publisher American Association for the Advancement of Science
Language eng
Abstract The finding that the metazoan hypoxic response is regulated by oxygen-dependent posttranslational hydroxylations, which regulate the activity and lifetime of hypoxia-inducible factor (HIF), has raised the question of whether other hydroxylases are involved in the regulation of gene expression. We reveal that the splicing factor U2 small nuclear ribonucleoprotein auxiliary factor 65-kilodalton subunit (U2AF65) undergoes posttranslational lysyl-5-hydroxylation catalyzed by the Fe(II) and 2-oxoglutarate–dependent dioxygenase Jumonji domain-6 protein (Jmjd6). Jmjd6 is a nuclear protein that has an important role in vertebrate development and is a human homolog of the HIF asparaginyl-hydroxylase. Jmjd6 is shown to change alternative RNA splicing of some, but not all, of the endogenous and reporter genes, supporting a specific role for Jmjd6 in the regulation of RNA splicing.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Faculty of Health and Behavioural Sciences -- Publications
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Created: Sun, 30 Oct 2011, 20:06:20 EST by Celia Webby on behalf of Faculty Of Health Sciences