Prenatal determination of fetal RhD type by DNA amplification

Bennett, Phillip R. , Kim, Caroline Le, Colin, Yves, Warwick, Ruth M. , Cherifzahar, Baya, Fisk, Nicholas M. and Cartron, Jean-Pierre (1993) Prenatal determination of fetal RhD type by DNA amplification. New England Journal of Medicine, 329 9: 607-610. doi:10.1056/NEJM199308263290903

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Author Bennett, Phillip R.
Kim, Caroline Le
Colin, Yves
Warwick, Ruth M.
Cherifzahar, Baya
Fisk, Nicholas M.
Cartron, Jean-Pierre
Title Prenatal determination of fetal RhD type by DNA amplification
Journal name New England Journal of Medicine   Check publisher's open access policy
ISSN 0028-4793
Publication date 1993-08-26
Year available 1993
Sub-type Article (original research)
DOI 10.1056/NEJM199308263290903
Open Access Status File (Publisher version)
Volume 329
Issue 9
Start page 607
End page 610
Total pages 4
Place of publication Waltham, MA, United States
Publisher Massachusetts Medical Society
Language eng
Formatted abstract
Background: An RhD-negative woman whose partner is heterozygous may have preexisting anti-RhD antibodies that may or may not affect a subsequent fetus, depending on whether it is heterozygous. A safe method of determining fetal RhD type early in pregnancy would eliminate the risks to an RhD-negative fetus of fetal-blood sampling or serial amniocenteses.

Methods: We determined the RhD type in 15 fetuses using the polymerase chain reaction in amniotic cells and serologic methods in fetal blood collected simultaneously. In another 15 fetuses, the RhD type determined from chorionic-villus samples was compared with that identified by typing of DNA from the fetus itself.

Results: RhD typing of DNA from amniotic cells correctly indicated the serologic type in every fetus. Of 10 fetuses with RhD-negative mothers, 4 were identified as RhD-negative and 6 as RhD-positive. Of five fetuses with RhD-positive mothers, four were identified as RhD-positive and one as RhD-negative. There was also complete agreement between the results of RhD typing of DNA from chorionic-villus samples and the results of typing of DNA from fetal tissue. Eleven fetuses were RhD-positive, and four were RhD-negative. Four RhD-positive fetuses had RhD-negative mothers. Three RhD-negative fetuses had RhD-positive mothers. There was no contamination by maternal RhD-positive DNA of the samples from RhD-negative fetuses.

Conclusions: Determining fetal RhD type in amniotic cells without invading the fetomaternal circulation is a reliable method that will be valuable in the management of Rh alloimmunization.
Keyword Medicine, General & Internal
General & Internal Medicine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 201 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 201 times in Scopus Article | Citations
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