Permeability of human placenta and fetal membranes to thyrotropin-stimulating hormone in vitro

Bajoria, R and Fisk, NM (1998) Permeability of human placenta and fetal membranes to thyrotropin-stimulating hormone in vitro. Pediatric Research, 43 5: 621-628. doi:10.1203/00006450-199805000-00010


Author Bajoria, R
Fisk, NM
Title Permeability of human placenta and fetal membranes to thyrotropin-stimulating hormone in vitro
Journal name Pediatric Research   Check publisher's open access policy
ISSN 0031-3998
Publication date 1998-05-01
Year available 1998
Sub-type Article (original research)
DOI 10.1203/00006450-199805000-00010
Open Access Status DOI
Volume 43
Issue 5
Start page 621
End page 628
Total pages 8
Place of publication BALTIMORE
Publisher INT PEDIATRIC RESEARCH FOUNDATION, INC
Language eng
Abstract We determined the placental transfer of TSH in an in vitro model of dually perfused isolated lobule in 28 human term placentas by adding varying concentrations (5-60 mu IU mL(-1)) of TSH as a single bolus dose to the closed maternal circulation. Transmembrane transfer of TSH was also studied by adding 45 mu IU mL(-1) to the maternal or fetal compartment of a dual chamber of fetal membranes in culture. Passage of freely diffusible markers creatinine and antipyrine were also studied in this model. TSH concentration was measured by third generation chemiluminescence assay with a sensitivity of 10 mIU mL(-1). In the perfusion experiments, at physiologic concentrations the slow decline of TSH in the maternal circulation was associated with a small linear increase in fetal levels to 0.11 +/- 0.04% of initial dose at 2 h. The placental transfer rate was 0.08 mu IU min(-1). Increasing maternal concentrations of TSH were associated with proportional increases in transfer rate (y = 0.002x; R-2 = 0.99) and placental uptake (y = 0.01x; R-2 = 0.97). The placental permeability of TSH was 2.4 . 10(-4) mL min(-1) g(-1) and was proportional to its coefficients of diffusion in water and molecular size. The transmembrane transfer and permeability of TSH was comparable to those of the placenta. We conclude that TSH crosses the human term placenta and fetal membranes sparingly.
Keyword Amniotic-Fluid Concentrations
Perfused Human-Placenta
Necrosis-Factor-Alpha
Transport
Lobule
Interleukin-6
Metabolism
Creatinine
Prolactin
Binding
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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