Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow

Campagnoli, Cesare, Roberts, Irene A. G., Kumar, Sailesh, Bennett, Phillip R., Bellantuono, Ilaria and Fisk, Nicholas M. (2001) Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow. Blood, 98 8: 2396-2402. doi:10.1182/blood.V98.8.2396


Author Campagnoli, Cesare
Roberts, Irene A. G.
Kumar, Sailesh
Bennett, Phillip R.
Bellantuono, Ilaria
Fisk, Nicholas M.
Title Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow
Journal name Blood   Check publisher's open access policy
ISSN 0006-4971
1528-0020
Publication date 2001-10-01
Year available 2001
Sub-type Article (original research)
DOI 10.1182/blood.V98.8.2396
Open Access Status
Volume 98
Issue 8
Start page 2396
End page 2402
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society of Hematology
Language eng
Formatted abstract
Human mesenchymal stem/progenitor cells (MSCs) have been identified in adult bone marrow, but little is known about their presence during fetal life. MSCs were isolated and characterized in first-trimester fetal blood, liver, and bone marrow. When 10 6 fetal blood nucleated cells (median gestational age, 10 +2 weeks [10 weeks, 2 days]) were cultured in 10% fetal bovine serum, the mean number (± SEM) of adherent fibroblastlike colonies was 8.2 ± 0.6/10 6 nucleated cells (69.6 ± 10/ μL fetal blood). Frequency declined with advancing gestation. Fetal blood MSCs could be expanded for at least 20 passages with a mean cumulative population doubling of 50.3 ± 4.5. In their undifferentiated state, fetal blood MSCs were CD29+, CD44 +, SH2 +, SH3 +, and SH4 +; produced prolyl-4-hydroxylase, α-smooth muscle actin, fibronectin, laminin, and vimentin; and were CD45 -, CD34 -, CD14 -, CD68 -, vWF -, and HLA-DR -. Fetal blood MSCs cultured in adipogenic, osteogenic, or chondrogenic media differentiated, respectively, into adipocytes, osteocytes, and chondrocytes. Fetal blood MSCs supported the proliferation and differentiation of cord blood CD34 + cells in longterm culture. MSCs were also detected in first-trimester fetal liver (11.3 ± 2.0/10 6 nucleated cells) and bone marrow (12.6 ± 3.6/10 6 nucleated cells). Their morphology, growth kinetics, and immunophenotype were comparable to those of fetal blood-derived MSCs and similarly differentiated along adipogenic, osteogenic, and chondrogenic lineages, even after sorting and expansion of a single mesenchymal cell. MSCs similar to those derived from adult bone marrow, fetal liver, and fetal bone marrow circulate in first-trimester human blood arid may provide novel targets for in utero cellular and gene therapy.
Keyword Stem-cells
Stromal cells
Progenitor cells
In-utero
Hematopoietic recovery
Human embryo
Yolk-sac
Transplantation
Differentiation
Precursors
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Health and Behavioural Sciences -- Publications
 
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