Identification, analysis, and linkage mapping of expressed sequence tags from the Australian sheep blowfly

Lee, Siu F., Chen, Zhenzhong, McGrath, Annette, Good, Robert T. and Batterham, Philip (2011) Identification, analysis, and linkage mapping of expressed sequence tags from the Australian sheep blowfly. BMC Genomics, 12 . doi:10.1186/1471-2164-12-406


Author Lee, Siu F.
Chen, Zhenzhong
McGrath, Annette
Good, Robert T.
Batterham, Philip
Title Identification, analysis, and linkage mapping of expressed sequence tags from the Australian sheep blowfly
Journal name BMC Genomics   Check publisher's open access policy
ISSN 1471-2164
Publication date 2011-08-01
Sub-type Article (original research)
DOI 10.1186/1471-2164-12-406
Open Access Status DOI
Volume 12
Total pages 13
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background
The Australian sheep blowfly Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae) is a destructive pest of the sheep, a model organism for insecticide resistance research, and a valuable tool for medical and forensic professionals. However, genomic information on L. cuprina is still sparse.

Results
We report here the construction of an embryonic and 2 larval cDNA libraries for L. cuprina. A total of 29,816 expressed sequence tags (ESTs) were obtained and assembled into 7,464 unique clusters. The sequence collection captures a great diversity of genes, including those related to insecticide resistance (e.g., 12 cytochrome P450s, 2 glutathione S transferases, and 6 esterases). Compared to Drosophila melanogaster, codon preference is different in 13 of the 18 amino acids encoded by redundant codons, reflecting the lower overall GC content in L. cuprina. In addition, we demonstrated that the ESTs could be converted into informative gene markers by capitalizing on the known gene structures in the model organism D. melanogaster. We successfully assigned 41 genes to their respective chromosomes in L. cuprina. The relative locations of these loci revealed high but incomplete chromosomal synteny between L. cuprina and D. melanogaster.

Conclusions
Our results represent the first major transcriptomic undertaking in L. cuprina. These new genetic resources could be useful for the blowfly and insect research community.
Keyword Cochliomyia-hominivorax diptera
Diazinon resistance genotypes
Maggot debridement therapy
Amino-acid substitution
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Biological Sciences Publications
 
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Created: Sun, 23 Oct 2011, 10:09:28 EST by System User on behalf of Aust Genome Research Facility