Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis

Klopffleisch, Karsten, Phan, Nguyen, Augustin, Kelsey, Bayne, Robert S., Booker, Katherine S., Botella, Jose R., Carpita, Nicholas C., Carr, Tyrell, Chen, Jin-Gui, Cooke, Thomas Ryan, Frick-Cheng, Arwen, Friedman, Erin J., Fulk, Brandon, Hahn, Michael G., Jiang, Kun, Jorda, Lucia, Kruppe, Lydia, Liu, Chenggang, Lorek, Justine, McCann, Maureen C., Molina, Antonio, Moriyama, Etsuko N., Mukhtar, M. Shahid, Mudgil, Yashwanti, Pattathil, Sivakumar, Schwarz, John, Seta, Steven, Tan, Matthew, Temp, Ulrike, Trusov, Yuri, Urano, Daisuke, Welter, Bastian, Yang, Jing, Panstruga, Ralph, Uhrig, Joachim F. and Jones, Alan M. (2011) Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis. Molecular Systems Biology, 7 532.1-532.7. doi:10.1038/msb.2011.66


Author Klopffleisch, Karsten
Phan, Nguyen
Augustin, Kelsey
Bayne, Robert S.
Booker, Katherine S.
Botella, Jose R.
Carpita, Nicholas C.
Carr, Tyrell
Chen, Jin-Gui
Cooke, Thomas Ryan
Frick-Cheng, Arwen
Friedman, Erin J.
Fulk, Brandon
Hahn, Michael G.
Jiang, Kun
Jorda, Lucia
Kruppe, Lydia
Liu, Chenggang
Lorek, Justine
McCann, Maureen C.
Molina, Antonio
Moriyama, Etsuko N.
Mukhtar, M. Shahid
Mudgil, Yashwanti
Pattathil, Sivakumar
Schwarz, John
Seta, Steven
Tan, Matthew
Temp, Ulrike
Trusov, Yuri
Urano, Daisuke
Welter, Bastian
Yang, Jing
Panstruga, Ralph
Uhrig, Joachim F.
Jones, Alan M.
Title Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis
Journal name Molecular Systems Biology   Check publisher's open access policy
ISSN 1744-4292
Publication date 2011-01-01
Year available 2011
Sub-type Article (original research)
DOI 10.1038/msb.2011.66
Open Access Status DOI
Volume 7
Start page 532.1
End page 532.7
Total pages 7
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract The heterotrimeric G-protein complex is minimally composed of G alpha, G beta, and G gamma subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G-protein effectors and scaffold proteins, we screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G-protein interactome. Within this core, over half of the interactions comprising two-thirds of the nodes were retested and validated as genuine in planta. Co-expression analysis in combination with phenotyping of loss-of-function mutations in a set of core interactome genes revealed a novel role for G-proteins in regulating cell wall modification. Molecular Systems Biology 7: 532; published online 27 September 2011; doi:10.1038/msb.2011.66
Formatted abstract
The heterotrimeric G-protein complex is minimally composed of Gα, Gβ, and Gγ subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G-protein effectors and scaffold proteins, we screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G-protein interactome. Within this core, over half of the interactions comprising two-thirds of the nodes were retested and validated as genuine in planta. Co-expression analysis in combination with phenotyping of loss-of-function mutations in a set of core interactome genes revealed a novel role for G-proteins in regulating cell wall modification.
Keyword AGB1
Arabidopsis
GPA1
Heterotrimeric G-proteins
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID MCB-0723515
DFG PA861/6-1
DBI-0923992
DE-AC05-00OR22725
Institutional Status UQ
Additional Notes Article # 532

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Agriculture and Food Sciences
 
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