Quantitative trait loci (QTL) mapping of resistance to strongyles and coccidia in the free-living Soay sheep (Ovis aries)

Beraldi, Dario, McRae, Allan F., Gratten, Jacob, Slate, Jon, Visscher, Peter M., Pemberton, Josephine M. and Pilkington, Jill G. (2007) Quantitative trait loci (QTL) mapping of resistance to strongyles and coccidia in the free-living Soay sheep (Ovis aries). Journal of Parasitology, 37 1: 121-129. doi:10.1016/j.ijpara.2006.09.007


Author Beraldi, Dario
McRae, Allan F.
Gratten, Jacob
Slate, Jon
Visscher, Peter M.
Pemberton, Josephine M.
Pilkington, Jill G.
Title Quantitative trait loci (QTL) mapping of resistance to strongyles and coccidia in the free-living Soay sheep (Ovis aries)
Formatted title
Quantitative trait loci (QTL) mapping of resistance to strongyles and coccidia in the free-living Soay sheep (Ovis aries)
Journal name Journal of Parasitology   Check publisher's open access policy
ISSN 0022-3395
1937-2345
Publication date 2007-01-01
Year available 2007
Sub-type Article (original research)
DOI 10.1016/j.ijpara.2006.09.007
Open Access Status DOI
Volume 37
Issue 1
Start page 121
End page 129
Total pages 9
Place of publication Lincoln, NE, United States
Publisher American Society of Parasitologists
Language eng
Subject 2405 Parasitology
2725 Infectious Diseases
Abstract A genome-wide scan was performed to detect quantitative trait loci (QTL) for resistance to gastrointestinal parasites and ectoparasitic keds segregating in the free-living Soay sheep population on St. Kilda (UK). The mapping panel consisted of a single pedigree of 882 individuals of which 588 were genotyped. The Soay linkage map used for the scans comprised 251 markers covering the whole genome at average spacing of 15 cM. The traits here investigated were the strongyle faecal egg count (FEC), the coccidia faecal oocyst count (FOC) and a count of keds (Melophagus ovinus). QTL mapping was performed by means of variance component analysis so that the genetic parameters of the study traits were also estimated and compared with previous studies in Soay and domestic sheep. Strongyle FEC and coccidia FOC showed moderate heritability (h(2) = 0.26 and 0.22, respectively) in lambs but low heritability in adults (h(2) < 0. 10). Ked count appeared to have very low h 2 in both lambs and adults. Genome scans were performed for the traits with moderate heritability and two genomic regions reached the level of suggestive linkage for coccidia FOC in lambs (logarithm of the odds = 2.68 and 2.21 on chromosomes 3 and X, respectively). We believe this is the first study to report a QTL search for parasite resistance in a free-living animal population and therefore may represent a useful reference for similar studies aimed at understanding the genetics of hostparasite co-evolution in the wild. (c) 2006 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
Formatted abstract
A genome-wide scan was performed to detect quantitative trait loci (QTL) for resistance to gastrointestinal parasites and ectoparasitic keds segregating in the free-living Soay sheep population on St. Kilda (UK). The mapping panel consisted of a single pedigree of 882 individuals of which 588 were genotyped. The Soay linkage map used for the scans comprised 251 markers covering the whole genome at average spacing of 15 cM. The traits here investigated were the strongyle faecal egg count (FEC), the coccidia faecal oocyst count (FOC) and a count of keds (Melophagus ovinus). QTL mapping was performed by means of variance component analysis so that the genetic parameters of the study traits were also estimated and compared with previous studies in Soay and domestic sheep. Strongyle FEC and coccidia FOC showed moderate heritability (h2 = 0.26 and 0.22, respectively) in lambs but low heritability in adults (h2 < 0.10). Ked count appeared to have very low h2 in both lambs and adults. Genome scans were performed for the traits with moderate heritability and two genomic regions reached the level of suggestive linkage for coccidia FOC in lambs (logarithm of the odds = 2.68 and 2.21 on chromosomes 3 and X, respectively). We believe this is the first study to report a QTL search for parasite resistance in a free-living animal population and therefore may represent a useful reference for similar studies aimed at understanding the genetics of host-parasite co-evolution in the wild.
Keyword QTL mapping
Soay sheep
Parasitic nematodes
Variance components
Natural population
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
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Created: Sat, 22 Oct 2011, 01:02:11 EST by Jake Gratten on behalf of Queensland Brain Institute