Specificity of synaptic connectivity between layer 1 inhibitory interneurons and layer 2/3 pyramidal neurons in the rat neocortex

Wozny, Christian and Williams, Stephen R. (2011) Specificity of synaptic connectivity between layer 1 inhibitory interneurons and layer 2/3 pyramidal neurons in the rat neocortex. Cerebral Cortex, 21 8: 1818-1826. doi:10.1093/cercor/bhq257


Author Wozny, Christian
Williams, Stephen R.
Title Specificity of synaptic connectivity between layer 1 inhibitory interneurons and layer 2/3 pyramidal neurons in the rat neocortex
Journal name Cerebral Cortex   Check publisher's open access policy
ISSN 1047-3211
1460-2199
Publication date 2011-01-01
Sub-type Article (original research)
DOI 10.1093/cercor/bhq257
Open Access Status DOI
Volume 21
Issue 8
Start page 1818
End page 1826
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Language eng
Formatted abstract
Understanding the structure and function of the neocortical microcircuit requires a description of the synaptic connectivity between identified neuronal populations. Here, we investigate the electrophysiological properties of layer 1 (L1) neurons of the rat
somatosensory neocortex (postnatal day 24--36) and their synaptic connectivity with supragranular pyramidal neurons. The active and passive properties of visually identified L1 neurons (n=266) suggested division into 4 groups according to the Petilla classification scheme with characteristics of neurogliaform cells (NGFCs) (n=72), classical-accommodating (n=137), fast-spiking (n=23), and burst-spiking neurons (n=34). Anatomical reconstructions of L1 neurons supported the existence of 4 major neuronal groups. Multiparameter unsupervised cluster analysis confirmed the existence of 4 groups, revealing a high degree of similarity with the Petilla scheme. Simultaneous recordings between synaptically connected L1 neurons and L2/3 pyramidal neurons (n=384) demonstrated neuronal class specificity in both
excitatory and inhibitory connectivity and the properties of synaptic potentials. Notably, all groups of L1 neurons received monosynaptic excitatory input from L2/3 pyramidal neurons (n=33), with the exception of NGFCs (n=68 pairs tested). In contrast, NGFCs strongly inhibited L2/3 pyramidal neurons (n=12 out 27 pairs tested). These data reveal a high specificity of excitatory and inhibitory connections in the superficial layers of the neocortex.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2012 Collection
 
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Created: Fri, 21 Oct 2011, 03:17:57 EST by Stephen Williams on behalf of Queensland Brain Institute