Major morphological sperm abnormalities in the bull are related to sperm DNA damage

Enciso, M., Cisale, H., Johnston, S.D., Sarasa, J., Fernandez, J.L. and Gosalvez, J. (2011) Major morphological sperm abnormalities in the bull are related to sperm DNA damage. Theriogenology, 76 1: 23-32. doi:10.1016/j.theriogenology.2010.12.034

Author Enciso, M.
Cisale, H.
Johnston, S.D.
Sarasa, J.
Fernandez, J.L.
Gosalvez, J.
Title Major morphological sperm abnormalities in the bull are related to sperm DNA damage
Journal name Theriogenology   Check publisher's open access policy
ISSN 0093-691X
Publication date 2011-07-01
Sub-type Article (original research)
DOI 10.1016/j.theriogenology.2010.12.034
Open Access Status Not Open Access
Volume 76
Issue 1
Start page 23
End page 32
Total pages 10
Place of publication United States
Publisher Elsevier Inc.
Language eng
Abstract The influence of sperm morphology and chromatin integrity on bull fertility suggests a strong but undefined biological relationship between these two parameters. In this study we explore this relationship, making use of the Sperm Chromatin Dispersion test, which allows simultaneous observation of sperm abnormalities and DNA fragmentation. Based on spermatozoa from 17 Holstein-Friesian bulls, we determined a relationship between DNA fragmentation and the presence of the "so called" major-type sperm defects. Values for DNA fragmentation index (mean ± SEM) calculated from cells with major-type abnormalities were significantly (P < 0.05) higher (85.05 ± 5.00%) than those from abnormal forms classified as minor-type (17.89 ± 5.55%). Some of the sperm abnormalities, such as double forms, narrow base heads, small heads, shortened tails and proximal cytoplasmic droplets, were only associated with sperm showing fragmented DNA. The simultaneous assessment of sperm morphology and DNA fragmentation has the potential to improve the efficacy of sperm quality assessment in this species.
Keyword Sperm DNA fragmentation
Sperm morphology
Major abnormalities
Minor abnormalities
Sperm Chromatin Dispersion
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Agriculture and Food Sciences
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Citation counts: TR Web of Science Citation Count  Cited 20 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 19 Oct 2011, 19:29:39 EST by Associate Professor Stephen Johnston on behalf of School of Agriculture and Food Sciences