Heteromerization of angiotensin receptors changes trafficking and arrestin recruitment profiles

Porrello, Enzo R., Pfleger, Kevin D. G., Seeber, Ruth M., Qian, Hongwei, Oro, Cristina, Abogadie, Fe, Delbridge, Lea M. D. and Thomas, Walter G. (2011) Heteromerization of angiotensin receptors changes trafficking and arrestin recruitment profiles. Cellular Signalling, 23 11: 1767-1776. doi:10.1016/j.cellsig.2011.06.011

Author Porrello, Enzo R.
Pfleger, Kevin D. G.
Seeber, Ruth M.
Qian, Hongwei
Oro, Cristina
Abogadie, Fe
Delbridge, Lea M. D.
Thomas, Walter G.
Title Heteromerization of angiotensin receptors changes trafficking and arrestin recruitment profiles
Journal name Cellular Signalling   Check publisher's open access policy
ISSN 0898-6568
Publication date 2011-11-01
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.cellsig.2011.06.011
Open Access Status Not Open Access
Volume 23
Issue 11
Start page 1767
End page 1776
Total pages 10
Place of publication Philadelphia, PA, U.S.A.
Publisher Elsevier
Language eng
Formatted abstract
The cardiovascular hormone angiotensin II (AngII) exerts its actions via two G protein-coupled receptor (GPCR) subtypes, AT1 and AT2, which often display antagonistic functions. Methodological constraints have so far precluded detailed analyses of the ligand-dependency, cellular localization, and functional relevance of AngII receptor interactions in live cells. In this study, we utilize a protein-fragment complementation assay (PCA) and GPCR-Heteromer Identification Technology (GPCR-HIT) to provide the first detailed investigation of the ligand-dependency and cellular localization of AngII receptor interactions in human embryonic kidney 293 cells. Fluorescent-tagged receptor constructs for PCA and GPCR-HIT displayed normal affinity and selectivity for AngII (AT1: IC50 = 1.0–1.6 nM; AT2: IC50 = 2.0–3.0 nM). Well-characterized angiotensin receptor interactions were used as positive and negative controls to demonstrate the sensitivity and specificity of these fluorescence-based assays. We report that AT1–AT2 receptor heteromers form constitutively, are localized to the plasma membrane and perinuclear compartments, and do not internalize following AngII stimulation despite arrestin being recruited specifically to the heteromer. Our findings using novel fluorescence-based technologies reveal a previously unrecognized mechanism of angiotensin receptor cross-talk involving cross-inhibition of AT1 receptor internalization through heteromerization with the AT2 receptor subtype.

Highlights: Novel fluorescence-based assays for studying angiotensin receptor interactions. ► AT1 and AT2 receptors form constitutive homo- and heteromers. ► AT2 receptor cross-inhibits AT1 receptor internalization. ► AT2 –AT1 heteromers do not co-internalize, despite b-Arrestin recruitment to dimer.
Keyword AT(1) receptor
AT(2) receptor
Protein-fragment complementation assay
Protein-coupled receptors
Energy-transfer BRET
II Type-1 receptor
Drug targets
AT1 receptor
AT2 receptor
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID FT100100271
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 24 times in Thomson Reuters Web of Science Article | Citations
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