A maternal diet supplemented with creatine from mid-pregnancy protects the newborn spiny mouse brain after birth hypoxia

Ireland, Z., Castillo-Melendez, M., Dickinson, H., Snow, R. and Walker, D.W. (2011) A maternal diet supplemented with creatine from mid-pregnancy protects the newborn spiny mouse brain after birth hypoxia. Neuroscience, 194 372-379. doi:10.1016/j.neuroscience.2011.05.012


Author Ireland, Z.
Castillo-Melendez, M.
Dickinson, H.
Snow, R.
Walker, D.W.
Title A maternal diet supplemented with creatine from mid-pregnancy protects the newborn spiny mouse brain after birth hypoxia
Journal name Neuroscience   Check publisher's open access policy
ISSN 0306-4522
1873-7544
Publication date 2011-10-27
Sub-type Article (original research)
DOI 10.1016/j.neuroscience.2011.05.012
Open Access Status Not Open Access
Volume 194
Start page 372
End page 379
Total pages 8
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Language eng
Abstract The creatine-phosphocreatine shuttle is essential for the maintenance of cellular ATP, particularly under hypoxic conditions when respiration may become anaerobic. Using a model of intrapartum hypoxia in the precocial spiny mouse (. Acomys cahirinus), the present study assessed the potential for maternal creatine supplementation during pregnancy to protect the developing brain from the effects of birth hypoxia. On day 38 of gestation (term is 39 days), the pregnant uterus was isolated and placed in a saline bath for 7.5 min, inducing global hypoxia. The pups were then removed, resuscitated, and cross-fostered to a nursing dam. Control offspring were delivered by caesarean section and recovered immediately after release from the uterus. At 24 h after birth hypoxia, the brains of offspring from dams fed a normal diet showed significant increases in lipid peroxidation as measured by the amount of malondialdehyde. In the cortical subplate, thalamus and piriform cortex there were significant increases in cellular expression of the pro-apoptotic protein BAX, cytoplasmic cytochrome c and caspase-3. When pregnant dams were fed the creatine supplemented diet, the increase in malondialdehyde, BAX, cytochrome c and caspase 3 were almost completely prevented, such that they were not different from control (caesarean-delivered) neonates. This study provides evidence that the neuroprotective capacity of creatine in the hypoxic perinatal brain involves abrogation of lipid peroxidation and apoptosis, possibly through the maintenance of mitochondrial function. Further investigation into these mechanisms of protection, and the long-term development and behavioural outcomes of such neonates is warranted.
Keyword Apoptosis
Birth hypoxia
Cytochrome c
Lipid peroxidation
Mitochondria
Perinatal brain damage
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Published in section: Regeneration, Repair, and Developmental Neuroscience Research Papers

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Medicine Publications
 
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Created: Sat, 15 Oct 2011, 03:09:17 EST by Ms Zoe Ireland on behalf of Paediatrics & Child Health - RBWH