Increasing electrospun scaffold pore size with tailored collectors for improved cell penetration

Vaquette, Cedryck and Cooper-White, Justin John (2011) Increasing electrospun scaffold pore size with tailored collectors for improved cell penetration. Acta Biomaterialia, 7 6: 2544-2557. doi:10.1016/j.actbio.2011.02.036


Author Vaquette, Cedryck
Cooper-White, Justin John
Title Increasing electrospun scaffold pore size with tailored collectors for improved cell penetration
Journal name Acta Biomaterialia   Check publisher's open access policy
ISSN 1742-7061
1878-7568
Publication date 2011-06-01
Sub-type Article (original research)
DOI 10.1016/j.actbio.2011.02.036
Open Access Status Not yet assessed
Volume 7
Issue 6
Start page 2544
End page 2557
Total pages 14
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Subject 1305 Biotechnology
1303 Biochemistry
2502 Biomaterials
2204 Biomedical Engineering
1312 Molecular Biology
Abstract This study investigates the use of patterned collectors to increase the pore size of electrospun scaffolds for enhanced cell infiltration. The morphology of the patterned scaffolds was investigated by scanning electron microscopy, which showed that the collector pattern was accurately mimicked by the electrospun fibres. We observed an enlargement in the pore size and in the pore size distribution compared with conventional electrospinning. Mechanical testing revealed that the mechanical properties could be tailored, to some extent, according to the patterning and that the patterned scaffolds were softer than standard electrospun scaffolds. When NIH 3T3 fibroblasts were seeded onto patterned collectors improved cell infiltration was observed. Cells were able to penetrate up to 250 μm into the scaffolds, compared with 30 μm for the standard scaffolds. This increase in the depth of infiltration occurred as early as 24 h post-seeding and remained constant over 7 days. Crown Copyright
Formatted abstract
This study investigates the use of patterned collectors to increase the pore size of electrospun scaffolds for enhanced cell infiltration. The morphology of the patterned scaffolds was investigated by scanning electron microscopy, which showed that the collector pattern was accurately mimicked by the electrospun fibres. We observed an enlargement in the pore size and in the pore size distribution compared with conventional electrospinning. Mechanical testing revealed that the mechanical properties could be tailored, to some extent, according to the patterning and that the patterned scaffolds were softer than standard electrospun scaffolds. When NIH 3T3 fibroblasts were seeded onto patterned collectors improved cell infiltration was observed. Cells were able to penetrate up to 250 μm into the scaffolds, compared with 30 μm for the standard scaffolds. This increase in the depth of infiltration occurred as early as 24 h post-seeding and remained constant over 7 days.
Keyword Electrospinning
Patterned collectors
Pore size
Cell penetration
Polycaprolactone
Uniaxially aligned arrays.
Biomedical applications
Nanofibers
Fibers
Infiltration
Polymer
Porosity
Bone
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Fri, 14 Oct 2011, 18:38:07 EST by Justin Cooper-White on behalf of Aust Institute for Bioengineering & Nanotechnology