Ultrafast microfluidic mixer with three-dimensional flow focusing for studies of biochemical kinetics

Gambin, Yann, Simonnet, Claire, Vandelinder, Virginia, Deniz, Ashok and Groisman, Alex (2010) Ultrafast microfluidic mixer with three-dimensional flow focusing for studies of biochemical kinetics. Lab On a Chip, 10 5: 598-609. doi:10.1039/b914174j

Author Gambin, Yann
Simonnet, Claire
Vandelinder, Virginia
Deniz, Ashok
Groisman, Alex
Title Ultrafast microfluidic mixer with three-dimensional flow focusing for studies of biochemical kinetics
Journal name Lab On a Chip   Check publisher's open access policy
ISSN 1473-0197
Publication date 2010-01-01
Year available 2009
Sub-type Article (original research)
DOI 10.1039/b914174j
Open Access Status Not yet assessed
Volume 10
Issue 5
Start page 598
End page 609
Total pages 12
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Abstract Studies of the kinetics of biochemical reactions, especially of folding of proteins and RNA, are important for understanding the function of biomolecules and processes in live cells. Many biochemical reactions occur rapidly and thus need to be triggered on very short time scales for their kinetics to be studied, which is often accomplished by mixing in a turbulent flow. More rapid and sample-efficient mixing is achieved in laminar flow in a microfluidic device, in which the sample is two-dimensionally (2D) focused to a thin sheet. Here we describe the design and operation of an ultrafast microfluidic mixer with three-dimensional (3D) flow focusing. The confinement of a 3D-focused sample to a narrow stream near the middle of a microchannel renders its velocity nearly uniform and makes it possible to monitor the reaction kinetics without exclusion of any parts of the sample. Hence, the sample consumption is substantially reduced and the fluorescence of the sample can be monitored without a confocal setup. Moreover, the 3D-focusing allows facile measurements of velocity of the sample with a high spatial resolution using a specially developed technique based on epi-fluorescence imaging. The data on the velocity vs. position are used to precisely calibrate the conversion between position and the reaction time, which is essential for accurate kinetic measurements. The device performs mixing on a 10 µs scale, which is comparable to that of the laminar mixers with 2D focusing. Unlike previous ultrafast laminar mixers, which were machined in hard materials, the present microfluidic device is made of a single cast of poly(dimethylsiloxane), PDMS, and is thus simpler and less expensive to manufacture.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes First published as an Advance Article on the web 16th December 2009

Document type: Journal Article
Sub-type: Article (original research)
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Created: Thu, 13 Oct 2011, 22:10:50 EST by Yann Gambin on behalf of Institute for Molecular Bioscience