Outbreak of angular leaf spot, caused by Xanthomonas fragariae, in a Queensland strawberry germplasm collection

Young, Anthony J., Marney, Thomas S., Herrington, Mark, Hutton, Don, Gomez, Apollo O., Villiers, Adam, Campbell, Paul R. and Geering, Andrew D. W. (2011) Outbreak of angular leaf spot, caused by Xanthomonas fragariae, in a Queensland strawberry germplasm collection. Australasian Plant Pathology, 40 3: 286-292. doi:10.1007/s13313-011-0045-y


Author Young, Anthony J.
Marney, Thomas S.
Herrington, Mark
Hutton, Don
Gomez, Apollo O.
Villiers, Adam
Campbell, Paul R.
Geering, Andrew D. W.
Title Outbreak of angular leaf spot, caused by Xanthomonas fragariae, in a Queensland strawberry germplasm collection
Formatted title
Outbreak of angular leaf spot, caused by Xanthomonas fragariae, in a Queensland strawberry germplasm collection
Journal name Australasian Plant Pathology   Check publisher's open access policy
ISSN 0815-3191
1448-6032
Publication date 2011-05-01
Year available 2011
Sub-type Article (original research)
DOI 10.1007/s13313-011-0045-y
Open Access Status Not yet assessed
Volume 40
Issue 3
Start page 286
End page 292
Total pages 7
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Language eng
Subject 1110 Plant Science
Abstract Strawberry (Fragaria (x) ananassa) plants exhibiting leaf lesions consistent with angular leaf spot (ALS, caused by Xanthomonas fragariae Kennedy and King 1962) were identified in the Queensland strawberry germplasm at Bundeberg in May 2010. Water suspensions of bacterial ooze tested positive using a previously described primer set. However, the slow growth rate of X. fragariae and the presence of a fast-growing, non-pathogenic, undescribed Xanthomonas species presented problems that were overcome by dilution plating and DNA sequence analysis. Sequencing of the gyrB locus of putative colonies of X. fragariae indicated 100% sequence similarity to other X. fragariae isolates. A new set of diagnostic primers for X. fragariae based on the gyrB locus is presented.
Formatted abstract
Strawberry (Fragaria (×) ananassa) plants exhibiting leaf lesions consistent with angular leaf spot (ALS, caused by Xanthomonas fragariae Kennedy and King 1962) were identified in the Queensland strawberry germplasm at Bundeberg in May 2010. Water suspensions of bacterial ooze tested positive using a previously described primer set. However, the slow growth rate of X. fragariae and the presence of a fast-growing, nonpathogenic, undescribed Xanthomonas species presented problems that were overcome by dilution plating and DNA sequence analysis. Sequencing of the gyrB locus of putative colonies of X. fragariae indicated 100% sequence similarity to other X. fragariae isolates. A new set of diagnostic primers for X. fragariae based on the gyrB locus is presented.
Keyword Diagnostic PCR
gyrB locus
Cultural properties
Strains
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Queensland Alliance for Agriculture and Food Innovation
 
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Created: Wed, 12 Oct 2011, 13:43:45 EST by System User on behalf of Qld Alliance for Agriculture and Food Innovation