Intramacrophage survival of uropathogenic Escherichia coli: differences between diverse clinical isolates and between mouse and human macrophages

Bokil, Nilesh J., Totsika, Makrina, Carey, Alison J., Stacey, Katryn J., Hancock, Viktoria, Saunders, Bernadette M., Ravasi, Timothy, Ulett, Glenn C., Schembri, Mark A. and Sweet, Matthew J. (2011) Intramacrophage survival of uropathogenic Escherichia coli: differences between diverse clinical isolates and between mouse and human macrophages. Immunobiology, 216 11: 1164-1171. doi:10.1016/j.imbio.2011.05.011


Author Bokil, Nilesh J.
Totsika, Makrina
Carey, Alison J.
Stacey, Katryn J.
Hancock, Viktoria
Saunders, Bernadette M.
Ravasi, Timothy
Ulett, Glenn C.
Schembri, Mark A.
Sweet, Matthew J.
Title Intramacrophage survival of uropathogenic Escherichia coli: differences between diverse clinical isolates and between mouse and human macrophages
Formatted title
Intramacrophage survival of uropathogenic Escherichia coli: differences between diverse clinical isolates and between mouse and human macrophages
Journal name Immunobiology   Check publisher's open access policy
ISSN 0171-2985
1878-3279
Publication date 2011-11-01
Sub-type Article (original research)
DOI 10.1016/j.imbio.2011.05.011
Volume 216
Issue 11
Start page 1164
End page 1171
Total pages 8
Place of publication Jena, Germany
Publisher Urban und Fischer
Language eng
Formatted abstract
Uropathogenic E. coli (UPEC) are the primary cause of urinary tract infections. Recent studies have demonstrated that UPEC can invade and replicate within epithelial cells, suggesting that this bacterial pathogen may occupy an intracellular niche within the host. Given that many intracellular pathogens target macrophages, we assessed the interactions between UPEC and macrophages. Colonization of the mouse bladder by UPEC strain CFT073 resulted in increased expression of myeloid-restricted genes, consistent with the recruitment of inflammatory macrophages to the site of infection. In in vitro assays, CFT073 was able to survive within primary mouse bone marrow-derived macrophages (BMM) up to 24h post-infection. Three additional well-characterized clinical UPEC isolates associated with distinct UTI symptomatologies displayed variable long-term survival within BMM. UPEC strains UTI89 and VR50, originally isolated from patients with cystitis and asymptomatic bacteriuria respectively, showed elevated bacterial loads in BMM at 24h post-infection as compared to CFT073 and the asymptomatic bacteriuria strain 83972. These differences did not correlate with differential effects on macrophage survival or initial uptake of bacteria. E. coli UTI89 localized to a Lamp1+ vesicular compartment within BMM. In contrast to survival within mouse BMM, intracellular bacterial loads of VR50 were low in both human monocyte-derived macrophages (HMDM) and in human T24 bladder epithelial cells. Collectively, these data suggest that some UPEC isolates may subvert macrophage anti-microbial pathways, and that host species differences may impact on intracellular UPEC survival.
Keyword Bacterial pathogen
Bladder infection
Host defence
Macrophage
Species differences
Toll-like receptor
UPEC
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes EMDS Special Issue: Systems Biology of Macrophages and Dentritic Cells in Health and Disease

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 32 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 11 Oct 2011, 23:41:08 EST by Matt Sweet on behalf of School of Chemistry & Molecular Biosciences