Nuclear factor one X regulates the development of multiple cellular populations in the postnatal cerebellum

Piper, Michael, Harris, Lachlan, Barry, Guy, Heng, Yee Hsieh Evelyn, Plachez, Celine, Gronostajski, Richard M. and Richards, Linda J. (2011) Nuclear factor one X regulates the development of multiple cellular populations in the postnatal cerebellum. The Journal of Comparative Neurology, 519 17: 3532-3548. doi:10.1002/cne.22721


Author Piper, Michael
Harris, Lachlan
Barry, Guy
Heng, Yee Hsieh Evelyn
Plachez, Celine
Gronostajski, Richard M.
Richards, Linda J.
Title Nuclear factor one X regulates the development of multiple cellular populations in the postnatal cerebellum
Journal name The Journal of Comparative Neurology   Check publisher's open access policy
ISSN 0021-9967
1096-9861
Publication date 2011-12-01
Year available 2011
Sub-type Article (original research)
DOI 10.1002/cne.22721
Open Access Status PMC
Volume 519
Issue 17
Start page 3532
End page 3548
Total pages 17
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Abstract Development of the cerebellum involves the coordinated proliferation, differentiation, maturation, and integration of cells from multiple neuronal and glial lineages. In rodent models, much of this occurs in the early postnatal period. However, our understanding of the molecular mechanisms that regulate this phase of cerebellar development remains incomplete. Here, we address the role of the transcription factor nuclear factor one X (NFIX), in postnatal development of the cerebellum. NFIX is expressed by progenitor cells within the external granular layer and by cerebellar granule neurons within the internal granule layer. Using NFIX(-/-) mice, we demonstrate that the development of cerebellar granule neurons and Purkinje cells within the postnatal cerebellum is delayed in the absence of this transcription factor. Furthermore, the differentiation of mature glia within the cerebellum, such as Bergmann glia, is also significantly delayed in the absence of NFIX. Collectively, the expression pattern of NFIX, coupled with the delays in the differentiation of multiple cell populations of the developing cerebellum in NFIX(-/-) mice, suggest a central role for NFIX in the regulation of cerebellar development, highlighting the importance of this gene for the maturation of this key structure. J. Comp. Neurol. 519:3532-3548, 2011. (C) 2011 Wiley-Liss, Inc.
Formatted abstract
Development of the cerebellum involves the coordinated proliferation, differentiation, maturation, and integration of cells from multiple neuronal and glial lineages. In rodent models, much of this occurs in the early postnatal period. However, our understanding of the molecular mechanisms that regulate this phase of cerebellar development remains incomplete. Here, we address the role of the transcription factor nuclear factor one X (NFIX), in postnatal development of the cerebellum. NFIX is expressed by progenitor cells within the external granular layer and by cerebellar granule neurons within the internal granule layer. Using NFIX−/− mice, we demonstrate that the development of cerebellar granule neurons and Purkinje cells within the postnatal cerebellum is delayed in the absence of this transcription factor. Furthermore, the differentiation of mature glia within the cerebellum, such as Bergmann glia, is also significantly delayed in the absence of NFIX. Collectively, the expression pattern of NFIX, coupled with the delays in the differentiation of multiple cell populations of the developing cerebellum in NFIX−/− mice, suggest a central role for NFIX in the regulation of cerebellar development, highlighting the importance of this gene for the maturation of this key structure.
Keyword Cerebellum
Transcription factor
NFIX
Cerebellar granule neurons
Bergmann glia
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID MP-1003462
RMG-HL080624
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2012 Collection
School of Biomedical Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 25 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 11 Oct 2011, 22:55:05 EST by Dr Michael Piper on behalf of School of Biomedical Sciences